High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C

W. H. Velander, J. L. Johnson, R. L. Page, C. G. Russell, A. Subramanian, T. D. Wilkins, F. C. Gwazdauskas, C. Pittius, W. N. Drohan

Research output: Contribution to journalArticle

137 Scopus citations

Abstract

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 μg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from human plasma. These studies provide evidence that γ-carboxylation can occur at high levels in the mammary gland of a pig.

Original languageEnglish (US)
Pages (from-to)12003-12007
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number24
DOIs
StatePublished - Dec 1 1992

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