High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat

Somayanda M. Impa, Amaranatha R. Vennapusa, Raju Bheemanahalli, David Sabela, Dan Boyle, Harkamal Walia, S. V.Krishna Jagadish

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved. Ten winter wheat genotypes were exposed to six different night-time temperatures (15–27°C) during flowering and grain-filling stages in controlled environment chambers. We identified the night-time temperature of 23oC as the critical threshold beyond which a consistent decline in yields and quality was observed. Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures. KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument. Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain–starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase. The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.

Original languageEnglish (US)
Pages (from-to)431-447
Number of pages17
JournalPlant Cell and Environment
Volume43
Issue number2
DOIs
StatePublished - Feb 1 2020

Keywords

  • confocal laser scanning microscopy
  • grain composition
  • high night-time temperature
  • starch metabolism
  • wheat

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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