TY - GEN
T1 - High-Performance Affinity Chromatography
T2 - Applications in Drug-Protein Binding Studies and Personalized Medicine
AU - Li, Zhao
AU - Beeram, Sandya R.
AU - Bi, Cong
AU - Suresh, D.
AU - Zheng, Xiwei
AU - Hage, David S.
N1 - Funding Information:
Portions of this work were supported by the NIH under grants R01 GM044931 and R01 DK069629, and the NSF/EPSCoR program under grant EPS-1004094. D.S. was supported by the University Grants Commission in India through a Raman Postdoctoral Fellowship.
Publisher Copyright:
© 2016 Elsevier Inc. All rights reserved.
PY - 2016
Y1 - 2016
N2 - The binding of drugs with proteins and other agents in serum is of interest in personalized medicine because this process can affect the dosage and action of drugs. The extent of this binding may also vary with a given disease state. These interactions may involve serum proteins, such as human serum albumin or α1-acid glycoprotein, or other agents, such as lipoproteins. High-performance affinity chromatography (HPAC) is a tool that has received increasing interest as a means for studying these interactions. This review discusses the general principles of HPAC and the various approaches that have been used in this technique to examine drug-protein binding and in work related to personalized medicine. These approaches include frontal analysis and zonal elution, as well as peak decay analysis, ultrafast affinity extraction, and chromatographic immunoassays. The operation of each method is described and examples of applications for these techniques are provided. The type of information that can be obtained by these methods is also discussed, as related to the analysis of drug-protein binding and the study of clinical or pharmaceutical samples.
AB - The binding of drugs with proteins and other agents in serum is of interest in personalized medicine because this process can affect the dosage and action of drugs. The extent of this binding may also vary with a given disease state. These interactions may involve serum proteins, such as human serum albumin or α1-acid glycoprotein, or other agents, such as lipoproteins. High-performance affinity chromatography (HPAC) is a tool that has received increasing interest as a means for studying these interactions. This review discusses the general principles of HPAC and the various approaches that have been used in this technique to examine drug-protein binding and in work related to personalized medicine. These approaches include frontal analysis and zonal elution, as well as peak decay analysis, ultrafast affinity extraction, and chromatographic immunoassays. The operation of each method is described and examples of applications for these techniques are provided. The type of information that can be obtained by these methods is also discussed, as related to the analysis of drug-protein binding and the study of clinical or pharmaceutical samples.
KW - Alpha-acid glycoprotein
KW - Drug-protein binding
KW - Glycation
KW - High-performance affinity chromatography
KW - Human serum albumin
KW - Lipoproteins
KW - Personalized medicine
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U2 - 10.1016/bs.apcsb.2015.09.007
DO - 10.1016/bs.apcsb.2015.09.007
M3 - Conference contribution
C2 - 26827600
AN - SCOPUS:84953326923
SN - 9780128047958
T3 - Advances in Protein Chemistry and Structural Biology
SP - 1
EP - 39
BT - Advances in Protein Chemistry and Structural Biology
A2 - Donev, Rossen
PB - Academic Press Inc.
ER -