High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans

Jacob M. Hornby; Raluca Dumitru; Kenneth W. Nickerson

doi:10.1016/j.mimet.2003.09.021

High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans

Jacob M. Hornby, Raluca Dumitru, Kenneth W. Nickerson

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37°C (ca. 5×10⁶ cells/ml) in a glucose-proline-N-acetyl- glucosamine medium supplemented with up to 600 mM phosphate (KH ₂PO₄/K₂HPO₄ 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.

Original language	English (US)
Pages (from-to)	119-124
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	56
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2003.09.021
State	Published - Jan 2004
Externally published	Yes

Keywords

Candida albicans
High phosphate
Pseudohyphae
Pseudomycelia

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2003.09.021

Cite this

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title = "High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans",

abstract = "A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37°C (ca. 5×106 cells/ml) in a glucose-proline-N-acetyl- glucosamine medium supplemented with up to 600 mM phosphate (KH 2PO4/K2HPO4 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.",

keywords = "Candida albicans, High phosphate, Pseudohyphae, Pseudomycelia",

author = "Hornby, {Jacob M.} and Raluca Dumitru and Nickerson, {Kenneth W.}",

note = "Funding Information: This work was supported by grants from the National Science Foundation (MCB-0110999) and the University of Nebraska Tobacco Settlement Biomedical Research Enhancement Fund. We thank Kit Lee for performing the scanning electron microscopy.",

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doi = "10.1016/j.mimet.2003.09.021",

language = "English (US)",

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T1 - High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans

AU - Hornby, Jacob M.

AU - Dumitru, Raluca

AU - Nickerson, Kenneth W.

N1 - Funding Information: This work was supported by grants from the National Science Foundation (MCB-0110999) and the University of Nebraska Tobacco Settlement Biomedical Research Enhancement Fund. We thank Kit Lee for performing the scanning electron microscopy.

PY - 2004/1

Y1 - 2004/1

N2 - A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37°C (ca. 5×106 cells/ml) in a glucose-proline-N-acetyl- glucosamine medium supplemented with up to 600 mM phosphate (KH 2PO4/K2HPO4 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.

AB - A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37°C (ca. 5×106 cells/ml) in a glucose-proline-N-acetyl- glucosamine medium supplemented with up to 600 mM phosphate (KH 2PO4/K2HPO4 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.

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KW - Pseudohyphae

KW - Pseudomycelia

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High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans

Abstract

Keywords

ASJC Scopus subject areas

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