While the apolipoproteins play a central biochemical role in lipid transportation and cardiovascular diseases, structural studies of the apolipoproteins have been primarily confined to optical spectroscopies (circular dichroism and fluorescence). Using deuterated dodecylphosphocholine (DPC) to model the lipoprotein environment, we report the acquisition of high resolution 1H NMR spectra for human apolipoprotein C-III2 isolated from the plasma of a type V hyperlipoproteinemic patient. At a peptide:DPC ratio of 1:60 the pKa of His18 was determined to be 6.15 suggesting the residue is solvent accessible.
|Original language||English (US)|
|Number of pages||8|
|Journal||Protein and Peptide Letters|
|State||Published - 1997|
ASJC Scopus subject areas
- Structural Biology