High resolution 1H NMR spectra of human apolipoprotein C-III2 using DPC to model the lipoprotein environment

Garry W. Buchko, Annett Rozek, Guangshun Wang, Jiri J. Frohlich, Robert J. Cushley

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


While the apolipoproteins play a central biochemical role in lipid transportation and cardiovascular diseases, structural studies of the apolipoproteins have been primarily confined to optical spectroscopies (circular dichroism and fluorescence). Using deuterated dodecylphosphocholine (DPC) to model the lipoprotein environment, we report the acquisition of high resolution 1H NMR spectra for human apolipoprotein C-III2 isolated from the plasma of a type V hyperlipoproteinemic patient. At a peptide:DPC ratio of 1:60 the pKa of His18 was determined to be 6.15 suggesting the residue is solvent accessible.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalProtein and Peptide Letters
Issue number1
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry


Dive into the research topics of 'High resolution <sup>1</sup>H NMR spectra of human apolipoprotein C-III<sub>2</sub> using DPC to model the lipoprotein environment'. Together they form a unique fingerprint.

Cite this