High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins

Michael G. Zeece, Douglas L. Holt, Randy L. Wehling, Michael B. Liewen, Lynne R. Bush

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


This work describes a high-resolution two-dimensional electrophoretic method for identification of bovine milk caseins. The isoelectric focusing separation was carried out in 100-jtL capillary tubes using high field strengths to achieve enhanced resolution. Subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed in a minigel format, and the result of the combined techniques gave enhanced protein resolution while requiring substantially less time than conventional two-dimensional methods. The procedure readily distinguished various isoforms of caseins, as well as phosphorylated forms of the proteins. The method was also used to demonstrate the effect of a proteinase derived from a Pseudomonas species on milk proteins.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number2
StatePublished - Mar 1 1989

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


Dive into the research topics of 'High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins'. Together they form a unique fingerprint.

Cite this