High-Resolution two-dimensional electrophoresis of wheat proteins

D. A. Dougherty, M. G. Zeece, R. L. Wehling, J. E. Partridge

Research output: Contribution to journalArticle

3 Scopus citations


A high-resolution two-dimensional electrophoretic for separating wheat endosperm proteins is described. Non-equilibrium pH gradient electrophoresis (NEPHGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) were used to examine proteins in non-fractioned extracts of wheat flours. NEPHGE was conducted in 100-μl capillary tubes at 3000 V, for a total of 4800 V h. The second dimension, SDS-PAGE, was performed as a slab mini-gel (70 × 80 × 1.0 mm) with constant application of 200 V. Image analysis of the two-dimensional separations of three wheat varieties showed approximately 35 and 41% homology of Centurk 78 and Rodeo varieties, respectively, when compared to a reference (Scout 66).

Original languageEnglish (US)
Pages (from-to)359-369
Number of pages11
JournalJournal of Chromatography A
Issue numberC
StatePublished - 1989

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'High-Resolution two-dimensional electrophoresis of wheat proteins'. Together they form a unique fingerprint.

  • Cite this

    Dougherty, D. A., Zeece, M. G., Wehling, R. L., & Partridge, J. E. (1989). High-Resolution two-dimensional electrophoresis of wheat proteins. Journal of Chromatography A, 480(C), 359-369. https://doi.org/10.1016/S0021-9673(01)84305-3