TY - JOUR
T1 - High-Resolution two-dimensional electrophoresis of wheat proteins
AU - Dougherty, D. A.
AU - Zeece, M. G.
AU - Wehling, R. L.
AU - Partridge, J. E.
PY - 1989
Y1 - 1989
N2 - A high-resolution two-dimensional electrophoretic for separating wheat endosperm proteins is described. Non-equilibrium pH gradient electrophoresis (NEPHGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) were used to examine proteins in non-fractioned extracts of wheat flours. NEPHGE was conducted in 100-μl capillary tubes at 3000 V, for a total of 4800 V h. The second dimension, SDS-PAGE, was performed as a slab mini-gel (70 × 80 × 1.0 mm) with constant application of 200 V. Image analysis of the two-dimensional separations of three wheat varieties showed approximately 35 and 41% homology of Centurk 78 and Rodeo varieties, respectively, when compared to a reference (Scout 66).
AB - A high-resolution two-dimensional electrophoretic for separating wheat endosperm proteins is described. Non-equilibrium pH gradient electrophoresis (NEPHGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) were used to examine proteins in non-fractioned extracts of wheat flours. NEPHGE was conducted in 100-μl capillary tubes at 3000 V, for a total of 4800 V h. The second dimension, SDS-PAGE, was performed as a slab mini-gel (70 × 80 × 1.0 mm) with constant application of 200 V. Image analysis of the two-dimensional separations of three wheat varieties showed approximately 35 and 41% homology of Centurk 78 and Rodeo varieties, respectively, when compared to a reference (Scout 66).
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U2 - 10.1016/S0021-9673(01)84305-3
DO - 10.1016/S0021-9673(01)84305-3
M3 - Article
AN - SCOPUS:0343536759
VL - 480
SP - 359
EP - 369
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -