High-Resolution two-dimensional electrophoresis of wheat proteins

D. A. Dougherty, M. G. Zeece, R. L. Wehling, J. E. Partridge

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A high-resolution two-dimensional electrophoretic for separating wheat endosperm proteins is described. Non-equilibrium pH gradient electrophoresis (NEPHGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) were used to examine proteins in non-fractioned extracts of wheat flours. NEPHGE was conducted in 100-μl capillary tubes at 3000 V, for a total of 4800 V h. The second dimension, SDS-PAGE, was performed as a slab mini-gel (70 × 80 × 1.0 mm) with constant application of 200 V. Image analysis of the two-dimensional separations of three wheat varieties showed approximately 35 and 41% homology of Centurk 78 and Rodeo varieties, respectively, when compared to a reference (Scout 66).

Original languageEnglish (US)
Pages (from-to)359-369
Number of pages11
JournalJournal of Chromatography A
Volume480
Issue numberC
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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