Abstract
A high-resolution two-dimensional electrophoretic for separating wheat endosperm proteins is described. Non-equilibrium pH gradient electrophoresis (NEPHGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) were used to examine proteins in non-fractioned extracts of wheat flours. NEPHGE was conducted in 100-μl capillary tubes at 3000 V, for a total of 4800 V h. The second dimension, SDS-PAGE, was performed as a slab mini-gel (70 × 80 × 1.0 mm) with constant application of 200 V. Image analysis of the two-dimensional separations of three wheat varieties showed approximately 35 and 41% homology of Centurk 78 and Rodeo varieties, respectively, when compared to a reference (Scout 66).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 359-369 |
| Number of pages | 11 |
| Journal | Journal of Chromatography A |
| Volume | 480 |
| Issue number | C |
| DOIs | |
| State | Published - 1989 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
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Dougherty, D. A., Zeece, M. G., Wehling, R. L., & Partridge, J. E. (1989). High-Resolution two-dimensional electrophoresis of wheat proteins. Journal of Chromatography A, 480(C), 359-369. https://doi.org/10.1016/S0021-9673(01)84305-3