High-speed image reconstruction for optically sectioned, super-resolution structured illumination microscopy

Zhaojun Wang, Tianyu Zhao, Huiwen Hao, Yanan Cai, Kun Feng, Xue Yun, Yansheng Liang, Shaowei Wang, Yujie Sun, Piero R. Bianco, Kwangsung Oh, Ming Lei

Research output: Contribution to journalArticlepeer-review

Abstract

Super-resolution structured illumination microscopy (SR-SIM) is an outstanding method for visualizing the subcellular dynamics in living cells. To date, by using elaborately designed systems and algorithms, SR-SIM can achieve rapid, optically sectioned, SR observation with hundreds to thousands of time points. However, real-time observation is still out of reach for most SIM setups as conventional algorithms for image reconstruction involve a heavy computing burden. To address this limitation, an accelerated reconstruction algorithm was developed by implementing a simplified workflow for SR-SIM, termed joint space and frequency reconstruction. This algorithm results in an 80-fold improvement in reconstruction speed relative to the widely used Wiener-SIM. Critically, the increased processing speed does not come at the expense of spatial resolution or sectioning capability, as demonstrated by live imaging of microtubule dynamics and mitochondrial tubulation.

Original languageEnglish (US)
Article number026003
JournalAdvanced Photonics
Volume4
Issue number2
DOIs
StatePublished - Mar 1 2022

Keywords

  • high-speed image reconstruction
  • live-cell imaging
  • microtubule dynamics
  • mitochondrial tubulation
  • real-time structured illumination microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomedical Engineering

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