High temperature dilute acid pretreatment of coastal Bermuda grass for enzymatic hydrolysis

Arthur P. Redding, Ziyu Wang, Deepak R. Keshwani, Jay J. Cheng

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


Dilute sulfuric acid was used to pretreat coastal Bermuda grass at high temperature prior to enzymatic hydrolysis. After both pretreatment and enzymatic hydrolysis processes, the highest yield of total sugars (combined xylose and glucose) was 97% of the theoretical value. The prehydrolyzate liquor was analyzed for inhibitory compounds (furfural, hydroxymethylfurfural (HMF)) in order to assess potential risk for inhibition during the following fermentation. Accounting for the formation of the inhibitory compounds, a pretreatment with 1.2% acid at 140 °C for 30. min with a total sugar yield of 94% of the theoretical value may be more favorable for fermentation. From this study, it can be concluded that dilute sulfuric acid pretreatment can be successfully applied to coastal Bermuda grass to achieve high yields of monomeric glucose and xylose with acceptable levels of inhibitory compound formation.

Original languageEnglish (US)
Pages (from-to)1415-1424
Number of pages10
JournalBioresource Technology
Issue number2
StatePublished - Jan 2011


  • Coastal Bermuda grass
  • Dilute sulfuric acid pretreatment
  • Enzymatic hydrolysis
  • Furfural
  • Hydroxymethylfurfural

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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