Histamine utilizes JAK-STAT pathway in regulating cytokine production

Natalia Osna; Kathleen Elliott; Oleg Chaika; Eric Patterson; Robert E. Lewis; Manzoor M. Khan

doi:10.1016/S1567-5769(01)00009-1

Histamine utilizes JAK-STAT pathway in regulating cytokine production

Natalia Osna, Kathleen Elliott, Oleg Chaika, Eric Patterson, Robert E. Lewis, Manzoor M. Khan

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Histamine shifts TH1/TH2 cytokine balance from TH1 to TH2 cytokines. The phosphorylation of STAT factors and their translocation to nucleus are important steps in the regulation of TH1/TH2 cytokine balance. This study was designed to investigate the effects of histamine on Janus kinases-signal transducers and activators of transcription (JAK-STAT) pathway. The splenocytes were treated with histamine in the presence or absence of JAK-STAT inhibitor, tyrphostin, activated with IFNγ for 30 min, and phosphorylated STAT1 was detected by immunoblotting. We found that histamine up-regulated the phosphorylation of STAT1 and tyrphostin prevented this phosphorylation. We then studied the effects of tyrphostin on histamine-mediated inhibition of IFNγ production and histamine-mediated stimulation of IL-5 and IL-10 production. Tyrphostin dose-dependently reversed the effects of histamine on IFNγ, IL-5 and IL-10 production, as evident by ELISA. These observations suggest that histamine regulated JAK-STAT signal transduction, which is involved in cytokine secretion.

Original language	English (US)
Pages (from-to)	759-762
Number of pages	4
Journal	International Immunopharmacology
Volume	1
Issue number	4
DOIs	https://doi.org/10.1016/S1567-5769(01)00009-1
State	Published - 2001
Externally published	Yes

Keywords

Cytokine
Histamine
JAK-STAT

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Pharmacology

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10.1016/S1567-5769(01)00009-1

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title = "Histamine utilizes JAK-STAT pathway in regulating cytokine production",

abstract = "Histamine shifts TH1/TH2 cytokine balance from TH1 to TH2 cytokines. The phosphorylation of STAT factors and their translocation to nucleus are important steps in the regulation of TH1/TH2 cytokine balance. This study was designed to investigate the effects of histamine on Janus kinases-signal transducers and activators of transcription (JAK-STAT) pathway. The splenocytes were treated with histamine in the presence or absence of JAK-STAT inhibitor, tyrphostin, activated with IFNγ for 30 min, and phosphorylated STAT1 was detected by immunoblotting. We found that histamine up-regulated the phosphorylation of STAT1 and tyrphostin prevented this phosphorylation. We then studied the effects of tyrphostin on histamine-mediated inhibition of IFNγ production and histamine-mediated stimulation of IL-5 and IL-10 production. Tyrphostin dose-dependently reversed the effects of histamine on IFNγ, IL-5 and IL-10 production, as evident by ELISA. These observations suggest that histamine regulated JAK-STAT signal transduction, which is involved in cytokine secretion.",

keywords = "Cytokine, Histamine, JAK-STAT",

author = "Natalia Osna and Kathleen Elliott and Oleg Chaika and Eric Patterson and Lewis, {Robert E.} and Khan, {Manzoor M.}",

note = "Funding Information: This work was supported by NIH grant 2R15 AI 32670-02.",

year = "2001",

doi = "10.1016/S1567-5769(01)00009-1",

language = "English (US)",

volume = "1",

pages = "759--762",

journal = "International Immunopharmacology",

issn = "1567-5769",

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T1 - Histamine utilizes JAK-STAT pathway in regulating cytokine production

AU - Osna, Natalia

AU - Elliott, Kathleen

AU - Chaika, Oleg

AU - Patterson, Eric

AU - Lewis, Robert E.

AU - Khan, Manzoor M.

N1 - Funding Information: This work was supported by NIH grant 2R15 AI 32670-02.

PY - 2001

Y1 - 2001

N2 - Histamine shifts TH1/TH2 cytokine balance from TH1 to TH2 cytokines. The phosphorylation of STAT factors and their translocation to nucleus are important steps in the regulation of TH1/TH2 cytokine balance. This study was designed to investigate the effects of histamine on Janus kinases-signal transducers and activators of transcription (JAK-STAT) pathway. The splenocytes were treated with histamine in the presence or absence of JAK-STAT inhibitor, tyrphostin, activated with IFNγ for 30 min, and phosphorylated STAT1 was detected by immunoblotting. We found that histamine up-regulated the phosphorylation of STAT1 and tyrphostin prevented this phosphorylation. We then studied the effects of tyrphostin on histamine-mediated inhibition of IFNγ production and histamine-mediated stimulation of IL-5 and IL-10 production. Tyrphostin dose-dependently reversed the effects of histamine on IFNγ, IL-5 and IL-10 production, as evident by ELISA. These observations suggest that histamine regulated JAK-STAT signal transduction, which is involved in cytokine secretion.

AB - Histamine shifts TH1/TH2 cytokine balance from TH1 to TH2 cytokines. The phosphorylation of STAT factors and their translocation to nucleus are important steps in the regulation of TH1/TH2 cytokine balance. This study was designed to investigate the effects of histamine on Janus kinases-signal transducers and activators of transcription (JAK-STAT) pathway. The splenocytes were treated with histamine in the presence or absence of JAK-STAT inhibitor, tyrphostin, activated with IFNγ for 30 min, and phosphorylated STAT1 was detected by immunoblotting. We found that histamine up-regulated the phosphorylation of STAT1 and tyrphostin prevented this phosphorylation. We then studied the effects of tyrphostin on histamine-mediated inhibition of IFNγ production and histamine-mediated stimulation of IL-5 and IL-10 production. Tyrphostin dose-dependently reversed the effects of histamine on IFNγ, IL-5 and IL-10 production, as evident by ELISA. These observations suggest that histamine regulated JAK-STAT signal transduction, which is involved in cytokine secretion.

KW - Cytokine

KW - Histamine

KW - JAK-STAT

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JO - International Immunopharmacology

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ER -

Histamine utilizes JAK-STAT pathway in regulating cytokine production

Abstract

Keywords

ASJC Scopus subject areas

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