Histamine utilizes JAK-STAT pathway in regulating cytokine production

Natalia Osna, Kathleen Elliott, Oleg Chaika, Eric Patterson, Robert E. Lewis, Manzoor M. Khan

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Histamine shifts TH1/TH2 cytokine balance from TH1 to TH2 cytokines. The phosphorylation of STAT factors and their translocation to nucleus are important steps in the regulation of TH1/TH2 cytokine balance. This study was designed to investigate the effects of histamine on Janus kinases-signal transducers and activators of transcription (JAK-STAT) pathway. The splenocytes were treated with histamine in the presence or absence of JAK-STAT inhibitor, tyrphostin, activated with IFNγ for 30 min, and phosphorylated STAT1 was detected by immunoblotting. We found that histamine up-regulated the phosphorylation of STAT1 and tyrphostin prevented this phosphorylation. We then studied the effects of tyrphostin on histamine-mediated inhibition of IFNγ production and histamine-mediated stimulation of IL-5 and IL-10 production. Tyrphostin dose-dependently reversed the effects of histamine on IFNγ, IL-5 and IL-10 production, as evident by ELISA. These observations suggest that histamine regulated JAK-STAT signal transduction, which is involved in cytokine secretion.

Original languageEnglish (US)
Pages (from-to)759-762
Number of pages4
JournalInternational Immunopharmacology
Issue number4
StatePublished - 2001
Externally publishedYes


  • Cytokine
  • Histamine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology


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