@article{51972cc37a3e405f94f0ad86f383fb20,
title = "HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages",
abstract = "Macrophages are key elements of the innate immune system. Their HIV-1 infection is a complex process that involves multiple interacting factors and various steps and is further altered by exposure of infected cells to methamphetamine (Meth), a common drug of abuse in people living with HIV. This is reflected by dynamic changes in the intracellular and secreted proteomes of these cells. Quantification of these changes poses a challenge for experimental design and associated analytics. In this study, we measured the effect of Meth on expression of intracellular and secreted galectins-1, -3, and -9 in HIV-1 infected human monocyte-derived macrophages (hMDM) using SWATH-MS, which was further followed by MRM targeted mass spectrometry validation. Cells were exposed to Meth either prior to or after infection. Our results are the first to perform comprehensive quantifications of galectins in primary hMDM cells during HIV-1 infection and Meth exposure a building foundation for future studies on the molecular mechanisms underlying cellular pathology of hMDM resulting from viral infection and a drug of abuse—Meth.",
keywords = "Galectins, HIV, Innate immunity, Macrophages, Multiple reaction monitoring, Quantitative proteomics",
author = "Kinga Grabowska and Katarzyna Macur and Sarah Zieschang and Lubaba Zaman and Nicole Haverland and Andrew Schissel and Brenda Morsey and Fox, {Howard S.} and Pawel Ciborowski",
note = "Funding Information: The authors would like to thank Ms. Robin Taylor for her assistance in editing of text and preparation of graphics for publication. The mass spectrometry instrumentation available in the Mass Spectrometry and Proteomics Core Facility of the UNMC was used to perform MS analyses. The UNMC Mass Spectrometry and Proteomics Core Facility, which is administrated through the Office of the Vice Chancellor for Research, and is supported by state funds from the Nebraska Research Initiative (NRI). The RNAseq was performed at the UNMC Genomics Core Facility, which receives partial support from the Nebraska Research Network in Functional Genomics NE-INBRE P20GM103427-14 and the Fred & Pamela Buffett Cancer Center (P30CA036727), and the resulting data were analyzed using bioinformatic pipeline from the UNMC Bioinformatics and Systems Biology Core. Funding Information: The mass spectrometry instrumentation available in the Mass Spectrometry and Proteomics Core Facility of the UNMC was used to perform MS analyses. The UNMC Mass Spectrometry and Proteomics Core Facility, which is administrated through the Office of the Vice Chancellor for Research, and is supported by state funds from the Nebraska Research Initiative (NRI). Funding Information: Financial support was provided by National Institutes of Health grants: R01 DA043258 and P30 MH062261. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = feb,
doi = "10.1007/s13365-021-01025-4",
language = "English (US)",
volume = "28",
pages = "99--112",
journal = "Journal of NeuroVirology",
issn = "1355-0284",
publisher = "Springer New York",
number = "1",
}