HIV-1 coat protein gp120-induced increases in levels of intrasynaptosomal calcium

Avindra Nath, Rodolfo A. Padua, Jonathan D. Geiger

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The effects of human immunodeficiency virus type-1 (HIV-1) coat protein gp120 on levels of intrasynaptosomal calcium ([Ca2+]i) were determined in rat cortical synaptosomes. gp120 at concentrations of ≥ 400 pM, significantly (P < 0.05) increased levels of [Ca2+]i. Treatment with 20 mM KCl, reduced the concentrations of gp120 necessary to produce significant (P < 0.001) increases in [Ca2+]i. gp120-evoked increases in [Ca2+]i were prevented either by treatment with dantrolene or by removal of extracellular calcium with BATPA. The peak levels of gp120-induced increases in [Ca2+]i were not affected by calcium channel blockers lanthanum and nicardipine, by glutamate receptor antagonists MK-801 and NBQX, or by removal of endogenous glutamate with glutamate dehydrogenase. gp120-induced [Ca2+]i increases in presynaptic terminals may play a role in HIV-mediated effects in the central nervous system.

Original languageEnglish (US)
Pages (from-to)200-206
Number of pages7
JournalBrain Research
Volume678
Issue number1-2
DOIs
StatePublished - Apr 24 1995
Externally publishedYes

Keywords

  • Calcium
  • Calcium channel blocker
  • Dantrolene
  • Glutamate
  • HIV-1
  • Synaptosome
  • gp120

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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