TY - JOUR
T1 - HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome
T2 - New insights into blood-brain barrier dysfunction for HIV-1-associated dementia
AU - Ricardo-Dukelow, Mary
AU - Kadiu, Irena
AU - Rozek, Wojciech
AU - Schlautman, Joshua
AU - Persidsky, Yuri
AU - Ciborowski, Pawel
AU - Kanmogne, Georgette D.
AU - Gendelman, Howard E.
N1 - Funding Information:
This work was funded by NIH grants 1T32 NS07488 (to M.R.-D. and H.E.G.), 2R37 NS36126 and 5 P01 MH64570–03 (H.E.G), 1 P01 NS043985–01 (H.E.G and Y.P), P20 RR15635–03 (to G.D.K., Y.P., P.C. and H.E.G.); MH-068214 (to G.D.K.), and R21 AA013846, RO1 AA015913 (to Y.P.).
PY - 2007/4
Y1 - 2007/4
N2 - Blood-brain barrier (BBB) compromise and transendothelial migration of HIV-infected leukocytes into the central nervous system (CNS) underlies the neuropathogenesis of HIV-1 infection. How this occurs is incompletely understood. We used a proteomic platform integrating difference gel electrophoresis and tandem mass spectrometry peptide sequencing to determine the effects that HIV-1-infected macrophages have on human brain microvascular endothelial cell (HBMEC) protein profiles. HIV-1 infected monocyte-derived macrophages (MDM) induced the upregulation of over 200 HBMEC proteins. These included metabolic, voltage-gated ion channels, heat shock, transport, cytoskeletal, regulatory, and calcium binding proteins. Results were validated by Western blot analysis. We conclude that HIV-1-infected MDM affect the HBMEC proteome and, in this way, affect BBB dysfunction and the development of HIV-1 CNS disease.
AB - Blood-brain barrier (BBB) compromise and transendothelial migration of HIV-infected leukocytes into the central nervous system (CNS) underlies the neuropathogenesis of HIV-1 infection. How this occurs is incompletely understood. We used a proteomic platform integrating difference gel electrophoresis and tandem mass spectrometry peptide sequencing to determine the effects that HIV-1-infected macrophages have on human brain microvascular endothelial cell (HBMEC) protein profiles. HIV-1 infected monocyte-derived macrophages (MDM) induced the upregulation of over 200 HBMEC proteins. These included metabolic, voltage-gated ion channels, heat shock, transport, cytoskeletal, regulatory, and calcium binding proteins. Results were validated by Western blot analysis. We conclude that HIV-1-infected MDM affect the HBMEC proteome and, in this way, affect BBB dysfunction and the development of HIV-1 CNS disease.
KW - 2D difference gel electrophoresis
KW - Human brain microvascular endothelial cells
KW - Human immunodeficiency virus
KW - Monocyte-derived macrophages
KW - Proteomics
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U2 - 10.1016/j.jneuroim.2007.01.004
DO - 10.1016/j.jneuroim.2007.01.004
M3 - Article
C2 - 17321604
AN - SCOPUS:33947680768
SN - 0165-5728
VL - 185
SP - 37
EP - 46
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -