HIV-1 Tat-mediated neurotoxicity in retinal cells

Nivedita Chatterjee; Shannon Callen; Gail M. Seigel; Shilpa J. Buch

doi:10.1007/s11481-011-9257-8

HIV-1 Tat-mediated neurotoxicity in retinal cells

Nivedita Chatterjee, Shannon Callen, Gail M. Seigel, Shilpa J. Buch

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The current study was aimed at investigating the effect of HIV-1 protein Tat on the retinal neurosensory cell line R28. Exposure of Tat resulted in induction of pro-inflammatory mediators such as CXCL10 and TNF-α in addition to the activation marker GFAP in these cells. Conditioned media from Tat-treated R28 cells was able to induce monocyte migration, an effect that was blocked by CXCR3 antagonist. Complementary studies in the HIV-1 Tat-transgenic mice, showed a complete absence of the nuclear layer and the outer photoreceptor segments of the retina with a concomitant increase in glial activation. These findings lend support to the observation in post-HAART era of increased incidence of immune response-mediated retinal degeneration. These findings have direct relevance to diseases such as immune response uveitis and patients recovering from CMV retinitis.

Original language	English (US)
Pages (from-to)	399-408
Number of pages	10
Journal	Journal of Neuroimmune Pharmacology
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/s11481-011-9257-8
State	Published - Sep 2011

Keywords

Chemokine
HIV
Retina

ASJC Scopus subject areas

Neuroscience (miscellaneous)
Immunology and Allergy
Immunology
Pharmacology

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10.1007/s11481-011-9257-8

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title = "HIV-1 Tat-mediated neurotoxicity in retinal cells",

abstract = "The current study was aimed at investigating the effect of HIV-1 protein Tat on the retinal neurosensory cell line R28. Exposure of Tat resulted in induction of pro-inflammatory mediators such as CXCL10 and TNF-α in addition to the activation marker GFAP in these cells. Conditioned media from Tat-treated R28 cells was able to induce monocyte migration, an effect that was blocked by CXCR3 antagonist. Complementary studies in the HIV-1 Tat-transgenic mice, showed a complete absence of the nuclear layer and the outer photoreceptor segments of the retina with a concomitant increase in glial activation. These findings lend support to the observation in post-HAART era of increased incidence of immune response-mediated retinal degeneration. These findings have direct relevance to diseases such as immune response uveitis and patients recovering from CMV retinitis.",

keywords = "Chemokine, HIV, Retina",

author = "Nivedita Chatterjee and Shannon Callen and Seigel, {Gail M.} and Buch, {Shilpa J.}",

note = "Funding Information: Acknowledgments The authors thank Dr. Sabita Roy, University of Minnesota for providing the HIV-1 Tat-transgenic mice eyeballs. The authors also thank the Confocal Laser Scanning Microscope Core Facility at the University of Nebraska Medical Center for providing assistance with confocal microscopy and the Nebraska Research Initiative and the Eppley Cancer Center for their support of the Core Facility. We thank Dr. Sitabhra Sinha for his help in generating Fig. 2 with MATLAB. NC was supported by the Indian Council of Medical Research Young Biomedical Scientist Fellowship while working at UNMC on this work. GMS is supported by R21CA127061, a departmental challenge grant from Research to Prevent Blindness and a Vision Research core infrastructure grant 1R24EY016662 and by U54CA143876 from the National Cancer Institute.",

year = "2011",

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doi = "10.1007/s11481-011-9257-8",

language = "English (US)",

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AU - Buch, Shilpa J.

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HIV-1 Tat-mediated neurotoxicity in retinal cells

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Keywords

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