HIV-1 transforms the monocyte plasma membrane proteome

Irena Kadiu, Tong Wang, Joshua D. Schlautman, Larisa Dubrovsky, Pawel Ciborowski, Michael Bukrinsky, Howard E. Gendelman

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


How HIV-1 affects the monocyte proteome is incompletely understood. We posit that one functional consequence of virus-exposure to the monocyte is the facilitation of protein transformation from the cytosol to the plasma membrane (PM). To test this, cell surface labeling with CyDye fluorophores followed by 2 dimensional differential in-gel electrophoresis (2D DIGE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed. Fifty three percent of HIV-1 induced proteins were PM associated. These were linked, in large measure, to cellular activation and oxidative stress. They included, but not limited to, biliverdin reductase, leukotriene hydrolase A4, heat shock protein 70, and cystatin B. HIV-1 induced PM protein translocation was associated with cathepsin B- and caspase 9, 3-dependent apoptosis. In contrast, PMA-treated monocytes bypassed caspase 3, 9 pathways and lead to cathepsin B-dependent necrosis. These results demonstrate that HIV-1 uniquely affects monocyte activation and oxidative stress. These do not affect viral infection dynamics but are linked to stress-induced cell death.

Original languageEnglish (US)
Pages (from-to)44-58
Number of pages15
JournalCellular Immunology
Issue number1
StatePublished - 2009


  • Apoptosis
  • HIV-1
  • Monocytes
  • Necrosis
  • Oxidative stress
  • Phorbol myristate acetate
  • Plasma membrane
  • Proteomics
  • Vitamin E

ASJC Scopus subject areas

  • Immunology


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