[HIV-1gp120 injures neurons by alteration of neuronal expressions of NR2B and PSD-95].

Wenbi Xiong, Jinyan Xie, Jianuo Liu, Huangui Xiong

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To investigate the mechanisms underlying HIV-1 envelope glycoprotein 120 (gp120)-associated neurotoxicity on rat cortical neuronal cultures. Experiments were carried out on primary cortical neuronal cultures prepared from fetal Sprague-Dawley fetal rats. Immunocytochemistry and Western blot techniques were employed to examine the changes of the expressions of N-methyl-D-asparate receptors 2B(NR2B) and postsynaptic density 95 (PSD-95) in cultured neurons in the presence and absence of gp120 in the culture media. Gp120-induced neurotoxicity was determined by MTT assay. MTT assay showed gp120 produced dose-dependent neuronal injury when added to the culture media and the gp120-induced neuronal injury was blocked by memantine, a specific NR2B receptor antagonist. Further studies revealed that gp120 induced neuronal injury by up-regulation of NR2B and down-regulation of PSD-95 expressions in rat cortical neurons. These results demonstrated that gp120 injures neurons via an increase of NR2B and a decrease of PSD-95 expressions. The gp120-induced neuronal injury can be blocked by a specific NR2B receptor antagonist, suggesting NR2B may function as a potential target for the development of therapeutic strategies.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Issue number2
StatePublished - Feb 2014

ASJC Scopus subject areas

  • General Medicine


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