HLA-B influences integrin beta-1 expression and pancreatic cancer cell migration

Bailee H. Sliker, Benjamin T. Goetz, Raina Barnes, Hannah King, H. Carlo Maurer, Kenneth P. Olive, Joyce C. Solheim

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Human leukocyte antigen (HLA) class I molecules present antigenic peptides to cytotoxic T cells, causing lysis of malignant cells. Transplantation biology studies have implicated HLA class I molecules in cell migration, but there has been little evidence presented that they influence cancer cell migration, a contributing factor in metastasis. In this study, we examined the effect of HLA-B on pancreatic cancer cell migration. HLA-B siRNA transfection increased the migration of the S2-013 pancreatic cancer cells but, in contrast, reduced migration of the PANC-1 and MIA PaCa-2 pancreatic cancer cell lines. Integrin molecules have previously been implicated in the upregulation of pancreatic cancer cell migration, and knockdown of HLA-B in S2-013 cells heightened the expression of integrin beta 1 (ITGB1), but in the PANC-1 and MIA PaCa-2 cells HLA-B knockdown diminished ITGB1 expression. A transmembrane sequence in an S2-013 HLA-B heavy chain matches a corresponding sequence in HLA-B in the BxPC-3 pancreatic cancer cell line, and knockdown of BxPC-3 HLA-B mimics the effect of S2-013 HLA-B knockdown on migration. In total, our findings indicate that HLA-B influences the expression of ITGB1 in pancreatic cancer cells, with concurrent distinctions in transmembrane sequences and effects on the migration of the cells.

Original languageEnglish (US)
Article number111960
JournalExperimental Cell Research
Issue number2
StatePublished - May 15 2020


  • HLA
  • Integrin
  • Major histocompatibility complex class I
  • Migration
  • Pancreatic cancer

ASJC Scopus subject areas

  • Cell Biology


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