@article{ab27a87350824d0dae0b867d8ec2754a,
title = "Homogeneous low-molecular-weight heparins with reversible anticoagulant activity",
abstract = "Low-molecular-weight heparins (LMWHs) are carbohydrate-based anticoagulants clinically used to treat thrombotic disorders, but impurities, structural heterogeneity or functional irreversibility can limit treatment options. We report a series of synthetic LMWHs prepared by cost-effective chemoenzymatic methods. The high activity of one defined synthetic LMWH against human factor Xa (FXa) was reversible in vitro and in vivo using protamine, demonstrating that synthetically accessible constructs can have a critical role in the next generation of LMWHs.",
author = "Yongmei Xu and Chao Cai and Kasemsiri Chandarajoti and Hsieh, {Po Hung} and Lingyun Li and Pham, {Truong Q.} and Sparkenbaugh, {Erica M.} and Juzheng Sheng and Key, {Nigel S.} and Rafal Pawlinski and Harris, {Edward N.} and Linhardt, {Robert J.} and Jian Liu",
note = "Funding Information: We thank J.E. Rogers for helpful discussions. This work is supported in part by US National Institutes of Health grants HL094463 (to J.L.), GM102137 (to J.L.), HL62244 (to R.J.L.), HL096972 (to R.J.L.), GM38060 (to R.J.L.) HL096679 (to R.P.) and HL117659 (to N.S.K. and R.P.). E.M.S. is supported by a US National Institutes of Health Research Training Grant (T32-HL007149). K.C. is a recipient of a Royal Thai Government fellowship.",
year = "2014",
month = apr,
doi = "10.1038/nchembio.1459",
language = "English (US)",
volume = "10",
pages = "248--250",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "4",
}