Human β-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response

Lindsey C. Pingel, Karl G. Kohlgraf, Christopher J. Hansen, Christopher G. Eastman, Deborah E. Dietrich, Kindra K. Burnell, Rupasree N. Srikantha, Xiangjun Xiao, Myriam Bélanger, Ann Progulske-Fox, Joseph E. Cavanaugh, Janet M. Guthmiller, Georgia K. Johnson, Sophie Joly, Zoya B. Kurago, Deborah V. Dawson, Kim A. Brogden

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Regulatory mechanisms in mucosal secretions and tissues recognize antigens and attenuate pro-inflammatory cytokine responses. Here, we asked whether human β-defensin 3 (HBD3) serves as an upstream suppressor of cytokine signaling that binds and attenuates pro-inflammatory cytokine responses to recombinant hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis strain 381. We found that HBD3 binds to immobilized rHagB and produces a significantly higher resonance unit signal in surface plasmon resonance spectroscopic analysis, than HBD2 and HBD1 that are used as control defensins. Furthermore, we found that HBD3 significantly attenuates (P<0.05) the interleukin (IL)-6, IL-10, granulocyte macrophage colony stimulating factor (GM-CSF) and tumor-necrosis factor-α (TNF-α) responses induced by rHagB in human myeloid dendritic cell culture supernatants and the extracellular signal-regulated kinases (ERK 1/2) response in human myeloid dendritic cell lysates. Thus, HBD3 binds rHagB and this interaction may be an important initial step to attenuate a pro-inflammatory cytokine response and an ERK 1/2 response.

Original languageEnglish (US)
Pages (from-to)643-649
Number of pages7
JournalImmunology and Cell Biology
Volume86
Issue number8
DOIs
StatePublished - Nov 1 2008

Keywords

  • Defensin
  • HBD3
  • Innate immunity
  • Pro-inflammatory cytokine suppression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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    Pingel, L. C., Kohlgraf, K. G., Hansen, C. J., Eastman, C. G., Dietrich, D. E., Burnell, K. K., Srikantha, R. N., Xiao, X., Bélanger, M., Progulske-Fox, A., Cavanaugh, J. E., Guthmiller, J. M., Johnson, G. K., Joly, S., Kurago, Z. B., Dawson, D. V., & Brogden, K. A. (2008). Human β-defensin 3 binds to hemagglutinin B (rHagB), a non-fimbrial adhesin from Porphyromonas gingivalis, and attenuates a pro-inflammatory cytokine response. Immunology and Cell Biology, 86(8), 643-649. https://doi.org/10.1038/icb.2008.56