Human blood and marrow side population stem cell and Stro-1 positive bone marrow stromal cell numbers decline with age, with an increase in quality of surviving stem cells: Correlation with cytokines

S. K. Brusnahan, T. R. McGuire, J. D. Jackson, J. T. Lane, K. L. Garvin, B. J. O'Kane, A. M. Berger, S. R. Tuljapurkar, M. A. Kessinger, J. G. Sharp

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Hematological deficiencies increase with aging leading to anemias, reduced hematopoietic stress responses and myelodysplasias. This study tested the hypothesis that side population hematopoietic stem cells (SP-HSC) would decrease with aging, correlating with IGF-1 and IL-6 levels and increases in bone marrow fat. Marrow was obtained from the femoral head and trochanteric region of the femur at surgery for total hip replacement (N= 100). Whole trabecular marrow samples were ground in a sterile mortar and pestle and cellularity and fat content determined. Marrow and blood mononuclear cells were stained with Hoechst dye and the SP-HSC profiles acquired. Marrow stromal cells (MSC) were enumerated flow cytometrically employing the Stro-1 antibody, and clonally in the colony forming unit fibroblast (CFU-F) assay. Plasma levels of IGF-1 (ng/ml) and IL-6 (pg/ml) were measured by ELISA. SP-HSC in blood and bone marrow decreased with age but the quality of the surviving stem cells increased. MSC decreased non-significantly. IGF-1 levels (mean = 30.7, SEM = 2) decreased and IL-6 levels (mean = 4.4, SEM = 1) increased with age as did marrow fat (mean = 1.2. mm. fat/g, SEM = 0.04). There were no significant correlations between cytokine levels or fat and SP-HSC numbers. Stem cells appear to be progressively lost with aging and only the highest quality stem cells survive.

Original languageEnglish (US)
Pages (from-to)718-722
Number of pages5
JournalMechanisms of Ageing and Development
Volume131
Issue number11-12
DOIs
StatePublished - Nov 2010

Keywords

  • Aging
  • IGF-1
  • IL-6
  • Marrow fat
  • Stem cells

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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