Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family

Andrew Koff, Fred Cross, Alfred Fisher, Jill Schumacher, Katherine Leguellec, Michel Philippe, James M. Roberts

Research output: Contribution to journalArticlepeer-review

598 Scopus citations


A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.

Original languageEnglish (US)
Pages (from-to)1217-1228
Number of pages12
Issue number6
StatePublished - Sep 20 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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