Human herpesvirus type 8 DNA sequences in cell-free plasma and mononuclear cells of Kaposi's sarcoma patients

William J. Harrington, Omar Bagasra, Carlos E. Sosa, Lisa E. Bobroski, Marianna Baum, Xue Lan Wen, Lisa Cabral, Gerald E. Byrne, Roger J. Pomerantz, Charles Wood

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63 Scopus citations

Abstract

Human herpesvirus (HHV) type 8 has been detected in both classical and AIDS-related Kaposi's sarcoma, body-cavity lymphomas, and other types of tumors. HHV-8 has also been detected in DNA from peripheral blood mononuclear cells (PBMC) of some Kaposi's sarcoma patients and more readily in B cell fractions derived from panned cell subpopulations. Two patients were followed using several methods: in situ hybridization, solution-based polymerase chain reaction (PCR), and in situ PCR. HHV-8 was intermittently detected in plasma, and detection correlated with detection in PBMC. In situ PCR demonstrated HHV-8 sequences in both peripheral blood B lymphocytes and, to a lesser extent, T lymphocytes. HHV-8 may undergo periods of viremia while at other times it is undetectable and infects circulating B cells and some T cells.

Original languageEnglish (US)
Pages (from-to)1101-1105
Number of pages5
JournalJournal of Infectious Diseases
Volume174
Issue number5
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Harrington, W. J., Bagasra, O., Sosa, C. E., Bobroski, L. E., Baum, M., Wen, X. L., Cabral, L., Byrne, G. E., Pomerantz, R. J., & Wood, C. (1996). Human herpesvirus type 8 DNA sequences in cell-free plasma and mononuclear cells of Kaposi's sarcoma patients. Journal of Infectious Diseases, 174(5), 1101-1105. https://doi.org/10.1093/infdis/174.5.1101