Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges

Mary K. Estes; Khalil Ettayebi; Victoria R. Tenge; Kosuke Murakami; Umesh Karandikar; Shih Ching Lin; B. Vijayalakshmi Ayyar; Nicolas W. Cortes-Penfield; Kei Haga; Frederick H. Neill; Antone R. Opekun; James R. Broughman; Xi Lei Zeng; Sarah E. Blutt; Sue E. Crawford; Sasirekha Ramani; David Y. Graham; Robert L. Atmar

doi:10.3390/v11070638

Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges

Mary K. Estes, Khalil Ettayebi, Victoria R. Tenge, Kosuke Murakami, Umesh Karandikar, Shih Ching Lin, B. Vijayalakshmi Ayyar, Nicolas W. Cortes-Penfield, Kei Haga, Frederick H. Neill, Antone R. Opekun, James R. Broughman, Xi Lei Zeng, Sarah E. Blutt, Sue E. Crawford, Sasirekha Ramani, David Y. Graham, Robert L. Atmar

Research output: Contribution to journal › Review article › peer-review

99 Scopus citations

Abstract

Noroviruses, in the genus Norovirus, are a significant cause of viral gastroenteritis in humans and animals. For almost 50 years, the lack of a cultivation system for human noroviruses (HuNoVs) was a major barrier to understanding virus biology and the development of effective antiviral strategies. This review presents a historical perspective of the development of a cultivation system for HuNoVs in human intestinal epithelial cell cultures. Successful cultivation was based on the discovery of genetically-encoded host factors required for infection, knowledge of the site of infection in humans, and advances in the cultivation of human intestinal epithelial cells achieved by developmental and stem cell biologists. The human stem cell-derived enteroid cultivation system recapitulates the multicellular, physiologically active human intestinal epithelium, and allows studies of virus-specific replication requirements, evaluation of human host-pathogen interactions, and supports the pre-clinical assessment of methods to prevent and treat HuNoV infections.

Original language	English (US)
Article number	638
Journal	Viruses
Volume	11
Issue number	7
DOIs	https://doi.org/10.3390/v11070638
State	Published - Jul 2019
Externally published	Yes

Keywords

Human norovirus cultivation
Intestinal enteroids/organoids
Virus neutralization and inactivation

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v11070638

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Estes, M. K., Ettayebi, K., Tenge, V. R., Murakami, K., Karandikar, U., Lin, S. C., Ayyar, B. V., Cortes-Penfield, N. W., Haga, K., Neill, F. H., Opekun, A. R., Broughman, J. R., Zeng, X. L., Blutt, S. E., Crawford, S. E., Ramani, S., Graham, D. Y., & Atmar, R. L. (2019). Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges. Viruses, 11(7), Article 638. https://doi.org/10.3390/v11070638

Estes, MK, Ettayebi, K, Tenge, VR, Murakami, K, Karandikar, U, Lin, SC, Ayyar, BV, Cortes-Penfield, NW, Haga, K, Neill, FH, Opekun, AR, Broughman, JR, Zeng, XL, Blutt, SE, Crawford, SE, Ramani, S, Graham, DY & Atmar, RL 2019, 'Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges', Viruses, vol. 11, no. 7, 638. https://doi.org/10.3390/v11070638

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title = "Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges",

abstract = "Noroviruses, in the genus Norovirus, are a significant cause of viral gastroenteritis in humans and animals. For almost 50 years, the lack of a cultivation system for human noroviruses (HuNoVs) was a major barrier to understanding virus biology and the development of effective antiviral strategies. This review presents a historical perspective of the development of a cultivation system for HuNoVs in human intestinal epithelial cell cultures. Successful cultivation was based on the discovery of genetically-encoded host factors required for infection, knowledge of the site of infection in humans, and advances in the cultivation of human intestinal epithelial cells achieved by developmental and stem cell biologists. The human stem cell-derived enteroid cultivation system recapitulates the multicellular, physiologically active human intestinal epithelium, and allows studies of virus-specific replication requirements, evaluation of human host-pathogen interactions, and supports the pre-clinical assessment of methods to prevent and treat HuNoV infections.",

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author = "Estes, {Mary K.} and Khalil Ettayebi and Tenge, {Victoria R.} and Kosuke Murakami and Umesh Karandikar and Lin, {Shih Ching} and Ayyar, {B. Vijayalakshmi} and Cortes-Penfield, {Nicolas W.} and Kei Haga and Neill, {Frederick H.} and Opekun, {Antone R.} and Broughman, {James R.} and Zeng, {Xi Lei} and Blutt, {Sarah E.} and Crawford, {Sue E.} and Sasirekha Ramani and Graham, {David Y.} and Atmar, {Robert L.}",

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AU - Estes, Mary K.

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AU - Tenge, Victoria R.

AU - Murakami, Kosuke

AU - Karandikar, Umesh

AU - Lin, Shih Ching

AU - Ayyar, B. Vijayalakshmi

AU - Cortes-Penfield, Nicolas W.

AU - Haga, Kei

AU - Neill, Frederick H.

AU - Opekun, Antone R.

AU - Broughman, James R.

AU - Zeng, Xi Lei

AU - Blutt, Sarah E.

AU - Crawford, Sue E.

AU - Ramani, Sasirekha

AU - Graham, David Y.

AU - Atmar, Robert L.

PY - 2019/7

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N2 - Noroviruses, in the genus Norovirus, are a significant cause of viral gastroenteritis in humans and animals. For almost 50 years, the lack of a cultivation system for human noroviruses (HuNoVs) was a major barrier to understanding virus biology and the development of effective antiviral strategies. This review presents a historical perspective of the development of a cultivation system for HuNoVs in human intestinal epithelial cell cultures. Successful cultivation was based on the discovery of genetically-encoded host factors required for infection, knowledge of the site of infection in humans, and advances in the cultivation of human intestinal epithelial cells achieved by developmental and stem cell biologists. The human stem cell-derived enteroid cultivation system recapitulates the multicellular, physiologically active human intestinal epithelium, and allows studies of virus-specific replication requirements, evaluation of human host-pathogen interactions, and supports the pre-clinical assessment of methods to prevent and treat HuNoV infections.

AB - Noroviruses, in the genus Norovirus, are a significant cause of viral gastroenteritis in humans and animals. For almost 50 years, the lack of a cultivation system for human noroviruses (HuNoVs) was a major barrier to understanding virus biology and the development of effective antiviral strategies. This review presents a historical perspective of the development of a cultivation system for HuNoVs in human intestinal epithelial cell cultures. Successful cultivation was based on the discovery of genetically-encoded host factors required for infection, knowledge of the site of infection in humans, and advances in the cultivation of human intestinal epithelial cells achieved by developmental and stem cell biologists. The human stem cell-derived enteroid cultivation system recapitulates the multicellular, physiologically active human intestinal epithelium, and allows studies of virus-specific replication requirements, evaluation of human host-pathogen interactions, and supports the pre-clinical assessment of methods to prevent and treat HuNoV infections.

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Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges

Abstract

Keywords

ASJC Scopus subject areas

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