Human primary gastric dendritic cells induce a Th1 response to H. pylori

D. Bimczok; R. H. Clements; K. B. Waites; L. Novak; D. E. Eckhoff; P. J. Mannon; P. D. Smith; L. E. Smythies

doi:10.1038/mi.2010.10

Human primary gastric dendritic cells induce a Th1 response to H. pylori

D. Bimczok, R. H. Clements, K. B. Waites, L. Novak, D. E. Eckhoff, P. J. Mannon, P. D. Smith, L. E. Smythies

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

Adaptive CD4 T-cell responses are important in the pathogenesis of chronic Helicobacter pylori gastritis. However, the gastric antigen-presenting cells that induce these responses have not yet been identified. Here we show that dendritic cells (DCs) are present in the gastric mucosa of healthy subjects and are more prevalent and more activated in the gastric mucosa of H. pylori-infected subjects. H. pylori induced gastric DCs isolated from noninfected subjects to express increased levels of CD11c, CD86 and CD83, and to secrete proinflammatory cytokines, particularly interleukin (IL)-6 and IL-8. Importantly, gastric DCs pulsed with live H. pylori, but not control DCs, mediated T-cell secretion of interferon-γ. The ability of H. pylori to induce gastric DC maturation and stimulate gastric DC activation of Th1 cells implicates gastric DCs as initiators of the immune response to H. pylori.

Original language	English (US)
Pages (from-to)	260-269
Number of pages	10
Journal	Mucosal Immunology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1038/mi.2010.10
State	Published - May 2010
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Human primary gastric dendritic cells induce a Th1 response to H. pylori",

abstract = "Adaptive CD4 T-cell responses are important in the pathogenesis of chronic Helicobacter pylori gastritis. However, the gastric antigen-presenting cells that induce these responses have not yet been identified. Here we show that dendritic cells (DCs) are present in the gastric mucosa of healthy subjects and are more prevalent and more activated in the gastric mucosa of H. pylori-infected subjects. H. pylori induced gastric DCs isolated from noninfected subjects to express increased levels of CD11c, CD86 and CD83, and to secrete proinflammatory cytokines, particularly interleukin (IL)-6 and IL-8. Importantly, gastric DCs pulsed with live H. pylori, but not control DCs, mediated T-cell secretion of interferon-γ. The ability of H. pylori to induce gastric DC maturation and stimulate gastric DC activation of Th1 cells implicates gastric DCs as initiators of the immune response to H. pylori.",

author = "D. Bimczok and Clements, {R. H.} and Waites, {K. B.} and L. Novak and Eckhoff, {D. E.} and Mannon, {P. J.} and Smith, {P. D.} and Smythies, {L. E.}",

note = "Funding Information: This study was supported by the National Institutes of Health (DK-54495, AI-83539, AI-78217, DK-47322, DK-084063, RR-20136 and the Mucosal HIV and Immunobiology Center, DK-64400) and the Research Service of the Veterans Administration. We thank G Perez-Perez (New York University School of Medicine) and John Y Kao (University of Ann Arbor) for providing H. pylori bacteria, and Donna Crabb and Lynn Duffy (University of Alabama at Birmingham) for preparing H. pylori cultures.",

year = "2010",

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doi = "10.1038/mi.2010.10",

language = "English (US)",

volume = "3",

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AU - Bimczok, D.

AU - Clements, R. H.

AU - Waites, K. B.

AU - Novak, L.

AU - Eckhoff, D. E.

AU - Mannon, P. J.

AU - Smith, P. D.

AU - Smythies, L. E.

N1 - Funding Information: This study was supported by the National Institutes of Health (DK-54495, AI-83539, AI-78217, DK-47322, DK-084063, RR-20136 and the Mucosal HIV and Immunobiology Center, DK-64400) and the Research Service of the Veterans Administration. We thank G Perez-Perez (New York University School of Medicine) and John Y Kao (University of Ann Arbor) for providing H. pylori bacteria, and Donna Crabb and Lynn Duffy (University of Alabama at Birmingham) for preparing H. pylori cultures.

PY - 2010/5

Y1 - 2010/5

N2 - Adaptive CD4 T-cell responses are important in the pathogenesis of chronic Helicobacter pylori gastritis. However, the gastric antigen-presenting cells that induce these responses have not yet been identified. Here we show that dendritic cells (DCs) are present in the gastric mucosa of healthy subjects and are more prevalent and more activated in the gastric mucosa of H. pylori-infected subjects. H. pylori induced gastric DCs isolated from noninfected subjects to express increased levels of CD11c, CD86 and CD83, and to secrete proinflammatory cytokines, particularly interleukin (IL)-6 and IL-8. Importantly, gastric DCs pulsed with live H. pylori, but not control DCs, mediated T-cell secretion of interferon-γ. The ability of H. pylori to induce gastric DC maturation and stimulate gastric DC activation of Th1 cells implicates gastric DCs as initiators of the immune response to H. pylori.

AB - Adaptive CD4 T-cell responses are important in the pathogenesis of chronic Helicobacter pylori gastritis. However, the gastric antigen-presenting cells that induce these responses have not yet been identified. Here we show that dendritic cells (DCs) are present in the gastric mucosa of healthy subjects and are more prevalent and more activated in the gastric mucosa of H. pylori-infected subjects. H. pylori induced gastric DCs isolated from noninfected subjects to express increased levels of CD11c, CD86 and CD83, and to secrete proinflammatory cytokines, particularly interleukin (IL)-6 and IL-8. Importantly, gastric DCs pulsed with live H. pylori, but not control DCs, mediated T-cell secretion of interferon-γ. The ability of H. pylori to induce gastric DC maturation and stimulate gastric DC activation of Th1 cells implicates gastric DCs as initiators of the immune response to H. pylori.

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Human primary gastric dendritic cells induce a Th1 response to H. pylori

Abstract

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