@article{b59679c428e446f48e5eb34345b05955,
title = "Human primase hangs on the primer–template and Polα to facilitate primer termination",
author = "{Ji Lim1}, Ci and Tahirov, {Tahir H.}",
note = "Funding Information: In 2016, we prepared an RNA primer containing a 5′-triphosphate and demonstrated that it stays bound to p58 after the initiation of primer synthesis. On the basis of these data, we proposed that the primer–template–p58 complex coordinates several transactions within Polα–primase and at the replication fork. To examine this, we developed a new method for 3′-DNA–RNA-5′-triphosphate primer synthesis and used it to prepare primosome complexes for X-ray crystallography structural determination. We got tiny needle-like crystals that were frustratingly time-consuming to improve. Then, in 2020, we began collaborating with Ci Ji Lim and his research group at the University of Wisconsin-Madison to use single-particle cryo-EM analysis to solve the structure of primosome DNA elongation complexes. The result of this fruitful collaboration is our reported cryo-EM structures of human primosome DNA elongation complexes. This work was supported by NIH NIGMS grants R00GM131023 to C.L. and R35GM127085 to T.H.T. T.H.T. C , C ",
year = "2023",
month = may,
doi = "10.1038/s41594-023-00972-2",
language = "English (US)",
volume = "30",
pages = "575--576",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Research",
number = "5",
}