Human T Cell Growth Factor (TCGF) produced by repeated stimulation of non-adherent human lymphocytes

Wu Yintang Wu, Ingemar Ernberg, Maria G. Masucci, Donald Johnson, Eva Klein, George Klein

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

T cell growth factor (TCGF) has become a valuable means of maintaining T lymphocytes in long-term culture and of studying T cell function. Numerous problems have been met in the production of TCGF of consistently good quality and in the maintenance of human T cell lines over long periods. We have investigated optimal conditions for TCGF production, and simplified assay systems for TCGF activity. The best TCGF production was obtained by short-term treatment with high concentrations of phytohemagglutinin (PHA). The TCGF producing lymphocytes could be re-used for TCGF production up to 1 month after the first treatment course. Human cultured T cell lines, fresh lymphocytes, short-term PHA stimulated lymphocytes and cultured marmoset T lymphocyte lines were all used for assay of TCGF. We recommend PHA stimulation of human lymphocytes for this assay on a routine basis, comparing results with a standard TCGF batch and calculating a growth index. Adherent cells impair TCGF production. Optimal TCGF production was seen when lymphocyte preparations without adherent cells from different donors were used.

Original languageEnglish (US)
Pages (from-to)35-44
Number of pages10
JournalJournal of Immunological Methods
Volume51
Issue number1
DOIs
Publication statusPublished - May 28 1982

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Keywords

  • PHA stimulation of TCGF
  • human T cell growth factor
  • production of TCGF

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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