Abstract
Specific TCR Vγ and Vδ segments are found to be coordinately used on subpopulations of γδ T lymphocytes. The reasons for this phenomenon are unknown, but may include the inability of particular chains expressing unique Vδ and Vγ segments to physically associate. Vδ2 is typically used together with Vγ2 on human peripheral blood γδ T lymphocytes. To examine whether Vδ2 can be used in conjunction with distinct Vγ segments, a TCR- mutant of the human γδ T cell line MOLT-13, which expresses parental TCR γ (Vγ1.3) but not TCR-δ protein, was transfected with plasmids containing full-length TCR-δ cDNA using either Vδ2 or Vδ3. TCR reconstitution was successful in both transfectants and resulted in TCR protein and RNA levels similar to that of the parental MOLT-13 cell line. These cell lines could be activated through their receptors as assessed by increases in cytoplasmic free calcium. These studies imply that physical constraints cannot explain the observed chain pairing preferences. Other possible explanations are discussed.
Original language | English (US) |
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Pages (from-to) | 1120-1126 |
Number of pages | 7 |
Journal | Journal of Immunology |
Volume | 144 |
Issue number | 3 |
State | Published - Feb 1 1990 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology