Hyaluronan synthesis in virus PBCV-1-infected chlorella-like green algae

Michael V. Graves, Dwight E. Burbank, Robyn Roth, John Heuser, Paul L. Deangelis, James L. Van Etten

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We previously reported that the chlorella virus PBCV-1 genome encodes an authentic, membrane-associated glycosyltransferase, hyaluronan synthase (HAS). Hyaluronan, a linear polysaccharide chain composed of alternating β1,4-glucuronic acid and β1,3-N-acetylglucosamine groups, is present in vertebrates as well as a few pathogenic bacteria. Studies of infected cells show that the transcription of the PBCV-1 has gene begins within 10 min of virus infection and ends at 60-90 min postinfection. The hyaluronan polysaccharide begins to accumulate as hyaluronan-lyase sensitive, hair-like fibers on the outside of the chlorella cell wall by 15-30 min postinfection; by 240 min postinfection, the infected cells are coated with a dense fibrous network. This hyaluronan slightly reduces attachment of a second chlorella virus to the infected algae. An analysis of 41 additional chlorella viruses indicates that many, but not all, produce hyaluronan during infection.

Original languageEnglish (US)
Pages (from-to)15-23
Number of pages9
JournalVirology
Volume257
Issue number1
DOIs
StatePublished - Apr 25 1999

Keywords

  • Chlorella virus
  • Glycosyltransferase
  • Hyaluronan synthase
  • PBCV-1
  • Phycodnaviridae
  • dsDNA virus

ASJC Scopus subject areas

  • Virology

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    Graves, M. V., Burbank, D. E., Roth, R., Heuser, J., Deangelis, P. L., & Van Etten, J. L. (1999). Hyaluronan synthesis in virus PBCV-1-infected chlorella-like green algae. Virology, 257(1), 15-23. https://doi.org/10.1006/viro.1999.9628