Hybrid resolution molecular dynamics simulations of amyloid proteins interacting with membranes

Mohtadin Hashemi, Yuri L. Lyubchenko

Research output: Contribution to journalArticlepeer-review

Abstract

A broad range of human diseases, including Alzheimer's and Parkinson's diseases, arise from or have as key players intrinsically disordered proteins. The aggregation of these amyloid proteins into fibrillar aggregates are the key events of such diseases. Characterizing the conformation dynamics of the proteins involved is crucial for understanding the molecular mechanisms of aggregation, which in turn is important for drug development efforts against these diseases. Computational approaches have provided extensive detail about some steps of the aggregation process, however the biologically relevant elements responsible for the aggregation and or aggregation propagation have not been fully characterized. Here we describe a hybrid resolution molecular dynamics simulation method that can be employed to investigate the interaction of amyloid proteins with lipid membranes, shown to dramatically accelerate the aggregation propensity of amyloid proteins. The hybrid resolution method enables routine and accurate simulation of multi-protein and complex membrane systems, mimicking biologically relevant lipid membranes, on microsecond time scales. The hybrid resolution method was applied to computer modeling of the interactions of α -synuclein protein with a mixed lipid bilayer.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - 2021

Keywords

  • Amyloid oligomer
  • Coarse-grained
  • Hybrid resolution
  • Lipid bilayer
  • Molecular dynamics
  • Neurodegeneration
  • Protein-lipid interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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