Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis

Surendra K. Shukla, Scott E. Mulder, Pankaj K. Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors that contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells. Here we describe an easy and simple way of measuring the hypoxia-mediated tumor glucose uptake in vivo. The method is based on fluorescent imaging probe, RediJect 2-DG, which is a nonradioactive fluorescent-tagged glucose molecule. We have discussed orthotopic tumor implantation of HIF-1α knockdown and control pancreatic cancer cells and glucose uptake measurement in vivo by using IVIS imaging system along with reagent preparations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-113
Number of pages7
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1742
ISSN (Print)1064-3745

Keywords

  • 2-DG
  • Cancer metabolism
  • Glucose uptake in mice tumors
  • HIF-1α
  • Hypoxia
  • In vivo glucose uptake

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Shukla, S. K., Mulder, S. E., & Singh, P. K. (2018). Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis. In Methods in Molecular Biology (pp. 107-113). (Methods in Molecular Biology; Vol. 1742). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7665-2_10