Ice nucleation in a Gram-positive bacterium isolated from precipitation depends on a polyketide synthase and non-ribosomal peptide synthetase

Kevin C. Failor, Haijie Liu, Marco E.Mechan Llontop, Sophie LeBlanc, Noam Eckshtain-Levi, Parul Sharma, Austin Reed, Shu Yang, Long Tian, Christopher T. Lefevre, Nicolas Menguy, Liangcheng Du, Caroline L. Monteil, Boris A. Vinatzer

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Earth’s radiation budget and frequency and intensity of precipitation are influenced by aerosols with ice nucleation activity (INA), i.e., particles that catalyze the formation of ice. Some bacteria, fungi, and pollen are among the most efficient ice nucleators but the molecular basis of INA is poorly understood in most of them. Lysinibacillus parviboronicapiens (Lp) was previously identified as the first Gram-positive bacterium with INA. INA of Lp is associated with a secreted, nanometer-sized, non-proteinaceous macromolecule or particle. Here a combination of comparative genomics, transcriptomics, and a mutant screen showed that INA in Lp depends on a type I iterative polyketide synthase and a non-ribosomal peptide synthetase (PKS-NRPS). Differential filtration in combination with gradient ultracentrifugation revealed that the product of the PKS-NRPS is associated with secreted particles of a density typical of extracellular vesicles and electron microscopy showed that these particles consist in “pearl chain”-like structures not resembling any other known bacterial structures. These findings expand our knowledge of biological INA, may be a model for INA in other organisms for which the molecular basis of INA is unknown, and present another step towards unraveling the role of microbes in atmospheric processes.

Original languageEnglish (US)
Pages (from-to)890-897
Number of pages8
JournalISME Journal
Volume16
Issue number3
DOIs
StatePublished - Mar 2022

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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