Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans

Maxine A. Lane; Ken W. Bayles; Ronald E. Yasbin

doi:10.1016/0378-1119(91)90371-H

Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans

Maxine A. Lane, Ken W. Bayles, Ronald E. Yasbin

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram^- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.

Original language	English (US)
Pages (from-to)	225-229
Number of pages	5
Journal	Gene
Volume	100
Issue number	C
DOIs	https://doi.org/10.1016/0378-1119(91)90371-H
State	Published - Apr 1991
Externally published	Yes

Keywords

Recombinant DNA
catabolite repression
dental caries
transcriptional fusions

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90371-H

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title = "Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans",

abstract = "Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.",

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author = "Lane, {Maxine A.} and Bayles, {Ken W.} and Yasbin, {Ronald E.}",

year = "1991",

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T1 - Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans

AU - Lane, Maxine A.

AU - Bayles, Ken W.

AU - Yasbin, Ronald E.

PY - 1991/4

Y1 - 1991/4

N2 - Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.

AB - Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.

KW - Recombinant DNA

KW - catabolite repression

KW - dental caries

KW - transcriptional fusions

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JO - Gene

JF - Gene

IS - C

ER -

Identification and initial characterization of glucose-repressible promoters of Streptococcus mutans

Abstract

Keywords

ASJC Scopus subject areas

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