Identification and quantitation of 7-(Benzo[a-]pyren-6-yl)guanine in the urine and feces of rats treated with benzo[a]pyrene

Eleanor G. Rogan, N. V.S. Ramakrishna, Sheila Higginbotham, Ercole L. Cavalieri, Hyuk Jeong, Ryszard Jankowiak, Gerald J. Small

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

The major identified benzo[o]pyrene (BP)-DNA adduct formed by cytochrome P-450 contains BP bound at the C-6 position to the N-7 position of guanine (BP-N7Gua). This adduct is rapidly depurinated from DNA. When rats were treated with [14C]BP, about 0.02% of the administered dose of BP was excreted as BP-N7Gua in feces and urine within 5 days. Chloroform extracts of the urine and feces were analyzed by high-pressure liquid chromatography. The structure of the adduct was established by cochromatography with electrochemically prepared BP-N7Gua and by fluorescence line narrowing spectrometry. This study represents the first demonstration that BP-N7Gua is formed in vivo in animals treated with BP.

Original languageEnglish (US)
Pages (from-to)441-444
Number of pages4
JournalChemical Research in Toxicology
Volume3
Issue number5
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Toxicology

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