Identification of a Ligand-Binding Site on the Staphylococcus aureus DnaG Primase C-Terminal Domain

Jonathan Catazaro, Jessica Periago, Matthew D. Shortridge, Bradley Worley, Andrew Kirchner, Robert Powers, Mark A. Griep

Research output: Contribution to journalArticlepeer-review

Abstract

The interface between the DnaG primase C-terminal domain (CTD) and the N-terminal domain of DnaB helicase is essential for bacterial DNA replication because it allows coordinated priming of DNA synthesis at the replication fork while the DNA is being unwound. Because these two proteins are conserved in all bacteria and distinct from those in eukaryotes, their interface is an attractive antibiotic target. To learn more about this interface, we determined the solution structure and dynamics of the DnaG primase CTD from Staphylococcus aureus, a medically important bacterial species. Comparison with the known primase CTD structures shows there are two biologically relevant conformations, an open conformation that likely binds to DnaB helicase and a closed conformation that does not. The S. aureus primase CTD is in the closed conformation, but nuclear magnetic resonance (NMR) dynamic studies indicate there is considerable movement in the linker between the two subdomains and that N564 is the most dynamic residue within the linker. A high-throughput NMR ligand affinity screen identified potential binding compounds, among which were acycloguanosine and myricetin. Although the affinity for these compounds and adenosine was in the millimolar range, all three bind to a common pocket that is present only on the closed conformation of the CTD. This binding pocket is at the opposite end of helices 6 and 7 from N564, the key hinge residue. The identification of this binding pocket should allow the development of stronger-binding ligands that can prevent formation of the CTD open conformation that binds to DnaB helicase.

Original languageEnglish (US)
Pages (from-to)932-943
Number of pages12
JournalBiochemistry
Volume56
Issue number7
DOIs
StatePublished - Feb 21 2017

ASJC Scopus subject areas

  • Biochemistry

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