Identification of a MAP65 isoform involved in directional expansion of plant cells

Jordi Chan, Guojie Mao, Andrei Smertenko, Patrick J. Hussey, Mike Naldrett, Andrew Bottrill, Clive W. Lloyd

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

MAP65 comprises a multigene family specific to plants. To see which isoform is utilised for the unique mechanism of cell expansion, uncomplicated by division structures, carrot cells were deprived of auxin whereupon they stopped dividing and elongated instead. During elongation, a MAP65 protein triplet reduced to a single band. Mass spectrometric analysis demonstrated that this corresponded to a single carrot cDNA; it also corresponded to the major protein previously shown to form filamentous cross-bridges between microtubules in vitro. This MAP65 isoform is concluded to have a major role in establishing the parallel microtubule arrays characteristic of cells undergoing directional expansion.

Original languageEnglish (US)
Pages (from-to)161-163
Number of pages3
JournalFEBS Letters
Volume534
Issue number1-3
DOIs
StatePublished - Jan 16 2003

Keywords

  • Cortical microtubule
  • MAP65
  • Plant cytoskeleton
  • Proteomics

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Chan, J., Mao, G., Smertenko, A., Hussey, P. J., Naldrett, M., Bottrill, A., & Lloyd, C. W. (2003). Identification of a MAP65 isoform involved in directional expansion of plant cells. FEBS Letters, 534(1-3), 161-163. https://doi.org/10.1016/S0014-5793(02)03848-6