Identification of a novel gene set in human cumulus cells predictive of an oocyte's pregnancy potential

Amy E. Iager, Arif M. Kocabas, Hasan H. Otu, Patricia Ruppel, Anna Langerveld, Patricia Schnarr, Mariluz Suarez, John C. Jarrett, Joe Conaghan, Guilherme J.M. Rosa, Emilio Fernández, Richard G. Rawlins, Jose B. Cibelli, Javier A. Crosby

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Objective: To identify a gene expression signature in human cumulus cells (CCs) predictive of pregnancy outcome across multiple clinics, taking into account the clinic and patient variations inherent in IVF practice. Design: Retrospective analysis of single human cumulus-oocyte complexes with the use of a combined microarray and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) approach. Setting: Multiple private IVF clinics. Patient(s): Fifty-eight patients. Samples from 55 patients underwent qRT-PCR analysis, and samples from 27 patients resulted in live birth. Intervention(s): Gene expression analysis for correlation with pregnancy outcome on individual human CCs collected immediately after oocyte retrieval. Pregnancy prediction analysis used leave-one-out cross-validation with weighted voting. Main Outcome Measure(s): Combinatorial expression of 12 genes in 101 samples from 58 patients. Result(s): We found a set of 12 genes predictive of pregnancy outcome based on their expression levels in CCs. This pregnancy prediction model had an accuracy of 78%, a sensitivity of 72%, a specificity of 84%, a positive predictive value of 81%, and a negative predictive value of 76%. Receiver operating characteristic analysis found an area under the curve of 0.763 ± 0.079, significantly greater than 0.5 (random chance prediction). Conclusion(s): Gene expression analysis in human CCs should be considered in identifying oocytes with a high potential to lead to pregnancy in IVF-ET.

Original languageEnglish (US)
Pages (from-to)745-752.e6
JournalFertility and Sterility
Issue number3
StatePublished - Mar 1 2013
Externally publishedYes


  • Human cumulus cells
  • gene expression
  • human embryo
  • human oocyte
  • pregnancy outcome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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