Identification of a tumor inhibitory factor in rat ascites fluid

Alan E. Levine, David A. Hamilton, Lynn C. Yeoman, Harris Busch, Michael G. Brattain

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


A polypeptide which inhibits the growth of human carcinoma cells has been characterized from Novikoff rat ascites fluid. This tumor inhibitory factor co-purified with transforming growth factor activity through acid/ethanol extraction and Bio-Gel chromatography. The two activities were completely separated by reverse phase HPLC. The tumor inhibitory factor is heat stable and requires disulfide bonds for bioactivity. This factor inhibited the anchorage independent growth of the more differentiated human colon carcinoma cell lines but did not affect the less differentiated carcinoma cells. The presence of stimulatory and inhibitory activities in the same extracts suggests that the relative concentrations of these factors may be important in the control of cell growth.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Feb 29 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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