Identification of an IgE-binding region in soybean acidic glycinin G1

Michael G. Zeece; Tom A. Beardslee; John P. Markwell; Gautam Sarath

doi:10.1080/09540109999942

Identification of an IgE-binding region in soybean acidic glycinin G1

Michael G. Zeece, Tom A. Beardslee, John P. Markwell, Gautam Sarath

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.

Original language	English (US)
Pages (from-to)	83-90
Number of pages	8
Journal	Food and Agricultural Immunology
Volume	11
Issue number	1
DOIs	https://doi.org/10.1080/09540109999942
State	Published - Mar 1999
Externally published	Yes

Keywords

Food allergy
Glycinin
IgE-binding proteins
Soybean

ASJC Scopus subject areas

Food Science
Immunology
Agronomy and Crop Science

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10.1080/09540109999942

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title = "Identification of an IgE-binding region in soybean acidic glycinin G1",

abstract = "The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.",

keywords = "Food allergy, Glycinin, IgE-binding proteins, Soybean",

author = "Zeece, {Michael G.} and Beardslee, {Tom A.} and Markwell, {John P.} and Gautam Sarath",

note = "Funding Information: A note of sincere thanks is extended to Ms Julie Nordlee and Ms April Kester for technical assistance and also to Dr Steve Taylor for the sera from soy-sensitive individuals. This research was supported in part by a grant from the Nebraska Soybean Association and by the Alliance for Food Protection.",

year = "1999",

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doi = "10.1080/09540109999942",

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T1 - Identification of an IgE-binding region in soybean acidic glycinin G1

AU - Zeece, Michael G.

AU - Beardslee, Tom A.

AU - Markwell, John P.

AU - Sarath, Gautam

N1 - Funding Information: A note of sincere thanks is extended to Ms Julie Nordlee and Ms April Kester for technical assistance and also to Dr Steve Taylor for the sera from soy-sensitive individuals. This research was supported in part by a grant from the Nebraska Soybean Association and by the Alliance for Food Protection.

PY - 1999/3

Y1 - 1999/3

N2 - The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.

AB - The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.

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KW - Glycinin

KW - IgE-binding proteins

KW - Soybean

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Identification of an IgE-binding region in soybean acidic glycinin G1

Abstract

Keywords

ASJC Scopus subject areas

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