Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses

Erin D. Scully, Teresa Donze-Reiner, Haichuan Wang, Thomas E. Eickhoff, Frederick Baxendale, Paul Twigg, Frank Kovacs, Tiffany Heng-Moss, Scott E. Sattler, Gautam Sarath

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Knowledge of specific peroxidases that respond to aphid herbivory is limited in C4 grasses, but could provide targets for improving defence against these pests. A sorghum (Sorghum bicolor (L.) Moench) peroxidase (SbPrx-1; Sobic.002G416700) has been previously linked to biotic stress responses, and was the starting point for this study. Genomic analyses indicated that SbPrx-1 was part of a clade of five closely related peroxidase genes occurring within a ∼30kb region on chromosome 2 of the sorghum genome. Comparison of this ∼30-kb region to syntenic regions in switchgrass (Panicum virgatum L.) and foxtail millet (Setaria italica L.) identified similar related clusters of peroxidases. Infestation of a susceptible sorghum cultivar with greenbugs (Shizaphis graminum Rondani) induced three of the five peroxidases. Greenbug infestation of switchgrass and foxtail millet plants showed similar inductions of peroxidases. SbPrx-1 was also induced in response to aphid herbivory in a greenbug-resistant sorghum line, Cargill 607E. These data indicate that this genomic region of C4 grasses could be valuable as a marker to assess potential insect resistance in C4 grasses.

Original languageEnglish (US)
Pages (from-to)1134-1148
Number of pages15
JournalFunctional Plant Biology
Volume43
Issue number12
DOIs
StatePublished - Jan 1 2016

Keywords

  • defence
  • defense
  • foxtail millet
  • greenbugs
  • plant resistance
  • sorghum
  • switchgrass
  • synteny.

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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    Scully, E. D., Donze-Reiner, T., Wang, H., Eickhoff, T. E., Baxendale, F., Twigg, P., Kovacs, F., Heng-Moss, T., Sattler, S. E., & Sarath, G. (2016). Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses. Functional Plant Biology, 43(12), 1134-1148. https://doi.org/10.1071/FP16104