TY - JOUR
T1 - Identification of neural progenitors in the adult mammalian eye
AU - Ahmad, Iqbal
AU - Tang, Lin
AU - Pham, Hao
N1 - Funding Information:
Thanks are due to Connie Dooley and Wally Thoreson for suggestions and critical reading of the manuscripts and Colin Barnstable and Roderick McInnes for RetP1 and Chx10 antibodies, respectively. This work was supported by the Foundation Fighting Blindness, Deans Indirect Cost Grant, University Nebraska Medical Center (UNMC), and Research to Prevent Blindness Grant to the Department of Ophthalmology (UNMC).
PY - 2000/4/13
Y1 - 2000/4/13
N2 - We have shown that the embryonic mammalian retina contains neural progenitors which display stem cell properties in vitro. Here we report the characterization of neural progenitors isolated from the adult mammalian eye. These quiescent cells, located in the pigmented ciliary bodies, proliferate in the presence of FGF2 and express the neuroectodermal marker nestin. The proliferating cells give rise to neural spheres and are multipotential; they express cell type-specific markers corresponding to neurons and glia. In addition, neural progenitors can generate secondary neural spheres, thus displaying potential to self-renew. The ciliary body-derived neural progenitors display retina-specific properties; the undifferentiated cells express Chx10, a retinal progenitor marker, and upon differentiation express markers corresponding to specific retinal cell types. Therefore, the pigmented ciliary body in the adult mammalian eye harbors neural progenitors that display stem cell properties and have the capacity to give rise to retinal neurons in vitro. (C) 2000 Academic Press.
AB - We have shown that the embryonic mammalian retina contains neural progenitors which display stem cell properties in vitro. Here we report the characterization of neural progenitors isolated from the adult mammalian eye. These quiescent cells, located in the pigmented ciliary bodies, proliferate in the presence of FGF2 and express the neuroectodermal marker nestin. The proliferating cells give rise to neural spheres and are multipotential; they express cell type-specific markers corresponding to neurons and glia. In addition, neural progenitors can generate secondary neural spheres, thus displaying potential to self-renew. The ciliary body-derived neural progenitors display retina-specific properties; the undifferentiated cells express Chx10, a retinal progenitor marker, and upon differentiation express markers corresponding to specific retinal cell types. Therefore, the pigmented ciliary body in the adult mammalian eye harbors neural progenitors that display stem cell properties and have the capacity to give rise to retinal neurons in vitro. (C) 2000 Academic Press.
UR - http://www.scopus.com/inward/record.url?scp=0034643271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034643271&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2000.2473
DO - 10.1006/bbrc.2000.2473
M3 - Article
C2 - 10753656
AN - SCOPUS:0034643271
SN - 0006-291X
VL - 270
SP - 517
EP - 521
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -