Identification of novel antioxidative peptides derived from a thermolytic Hydrolysate of ovotransferrin by LC-MS/MS

Gwen Shen, Baljot Chahal, Kaustav Majumder, Sun Jong You, Jianping Wu

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Ovotransferrin is a glycoprotein well-known for its iron-binding property. Ovotransferrin was reported to have antioxidative properties, but the presence of antioxidant peptides within the protein has not been reported. The purpose of the study was to characterize the antioxidant peptides within ovotransferrin. Ovotransferrin was sonicated and hydrolyzed by thermolysin, and peptides from the hydrolysate were fractionated by ion-exchange fast protein liquid chromatography and reversed-phase high-performance liquid chromatography. Fourteen peptides derived from ovotransferrin were characterized using LC-MS/MS, and their oxygen radical absorbance capacity (ORAC) values were determined using synthetic peptides. Two tetrapeptides (Trp-Asn-lle-Pro and Gly-Trp-Asn-lle) showed the highest antioxidant activity. Interestingly, the addition of amino acid residues to either the N or C terminus of the two peptides decreased the antioxidant activity, suggesting that the motif of Trp-Asn-lle is responsible for the high antioxidant activity.

Original languageEnglish (US)
Pages (from-to)7664-7672
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number13
DOIs
StatePublished - Jul 14 2010

Keywords

  • Antioxidant peptides
  • LC-MS/MS
  • Ovotransferrin
  • Thermolysin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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