@inproceedings{78a88123d7aa4e02833e05bd9b160a2e,
title = "Identification of pathogenic fungi using computational and molecular biological approaches",
abstract = "The lack of rapid diagnostic procedures is a major obstacle in the successful management of fungal disease. Most of the methods now used in the microbiology laboratory include growth-based phenotypic testing. However, Polymerase Chain Reaction (PCR) has allowed for rapid identification of organism without the need for a growing culture. An ion-paired reverse phase chromatography (IP-RP)-based assay using High performance liquid chromatography (HPLC) was developed with commercially available IP-RP-HPLC system called the WAVE to differentiate medically important fungi. Universal fungus-specific primers and Restriction Fragment Length Polymorphism (RFLP) of PCR amplicon were done prior to WAVE analysis. The assay was enhanced when combined with a searchable relational database of retention time. Discrimination among closely related fungal species was possible by evaluation of distinct-retention time patterns. This assay is simple, rapid, and allows for the identification of medically important fungi by searching the database with the information obtained from PCR-WAVE analysis.",
author = "Chandio, {Sarfraz H.} and Bastola, {Dhundy R.} and Iwen, {Peter C.} and Hinrichs, {Steven H.}",
year = "2005",
language = "English (US)",
isbn = "0780392329",
series = "2005 IEEE International Conference on Electro Information Technology",
booktitle = "2005 IEEE International Conference on Electro Information Technology",
note = "2005 IEEE International Conference on Electro Information Technology ; Conference date: 22-05-2005 Through 25-05-2005",
}