TY - JOUR
T1 - Identification of plakoglobin domains required for association with N-cadherin and α-catenin
AU - Sacco, Paula A.
AU - McGranahan, Tammy M.
AU - Wheelock, Margaret J.
AU - Johnson, Keith R.
PY - 1995/8/25
Y1 - 1995/8/25
N2 - Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, α-catenin, β-catenin, and γ-catenin (also known as plakoglobin), have been identified. The domain of the cadherin molecule important for its interaction with the catenins has been mapped to the COOH-terminal 70 amino acids, but less is known about regions of the catenins that allow them to associate with one another or with the cadherin molecule. In this study we have transfected carboxyl-terminal deletions of plakoglobin into the human fibrosarcoma HT-1080 and used immunofluorescence localization and co-immunoprecipitation to map the regions of plakoglobin that allow it to associate with N-cadherin and with α-catenin. Plakoglobin is an armadillo family member containing 13 weakly similar internal repeats. These data show that the α-catenin-binding region maps within the first repeat and the N-cadherin-binding region maps within repeats 7 and 8.
AB - Cadherins are calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. To function in cell-cell adhesion, the transmembrane cadherin molecule must be associated with the cytoskeleton via cytoplasmic proteins known as catenins. Three catenins, α-catenin, β-catenin, and γ-catenin (also known as plakoglobin), have been identified. The domain of the cadherin molecule important for its interaction with the catenins has been mapped to the COOH-terminal 70 amino acids, but less is known about regions of the catenins that allow them to associate with one another or with the cadherin molecule. In this study we have transfected carboxyl-terminal deletions of plakoglobin into the human fibrosarcoma HT-1080 and used immunofluorescence localization and co-immunoprecipitation to map the regions of plakoglobin that allow it to associate with N-cadherin and with α-catenin. Plakoglobin is an armadillo family member containing 13 weakly similar internal repeats. These data show that the α-catenin-binding region maps within the first repeat and the N-cadherin-binding region maps within repeats 7 and 8.
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U2 - 10.1074/jbc.270.34.20201
DO - 10.1074/jbc.270.34.20201
M3 - Article
C2 - 7650039
AN - SCOPUS:0029123863
SN - 0021-9258
VL - 270
SP - 20201
EP - 20206
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 34
ER -