TY - JOUR
T1 - Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1
AU - Powers, T. O.
AU - Szalanski, A. L.
AU - Mullin, P. G.
AU - Harris, T. S.
AU - Bertozzi, T.
AU - Griesbach, J. A.
PY - 2001/12
Y1 - 2001/12
N2 - A molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. Each anguinid nematode associated with a unique host produced a unique PCR-RFLP pattern for the ITS1 region. Anguina species that had been synonymized in the past, Anguina agrostis, A. funesta, and A. wevelli (Afrina wevwlli), were readily discriminated. Two undescribed species from northern New South Wales and southeastern South Australia, reported to be vectors of Rathyaibacter toxicus in the disease called "floodplain stagger," were differentiated by a single restriction enzyme, and both could be separated easily from A. funesta, the vector of R. toxicus in annual ryegrass toxicity. Other species differentiated in this study include A. agropyronifloris, A. graminis, A. microlaenae, A. pacificae, and undescrihed species from host species Dactylis glomerata, Agrostis avenacea, Polypogon monospeliensis, Stipa sp., Astrebla pectinata, and Holcus lanatus. Phylogenetic analysis of the ITS1 region suggests that considerable anguinid genetic diversification has accompanied specialization on different host species.
AB - A molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. Each anguinid nematode associated with a unique host produced a unique PCR-RFLP pattern for the ITS1 region. Anguina species that had been synonymized in the past, Anguina agrostis, A. funesta, and A. wevelli (Afrina wevwlli), were readily discriminated. Two undescribed species from northern New South Wales and southeastern South Australia, reported to be vectors of Rathyaibacter toxicus in the disease called "floodplain stagger," were differentiated by a single restriction enzyme, and both could be separated easily from A. funesta, the vector of R. toxicus in annual ryegrass toxicity. Other species differentiated in this study include A. agropyronifloris, A. graminis, A. microlaenae, A. pacificae, and undescrihed species from host species Dactylis glomerata, Agrostis avenacea, Polypogon monospeliensis, Stipa sp., Astrebla pectinata, and Holcus lanatus. Phylogenetic analysis of the ITS1 region suggests that considerable anguinid genetic diversification has accompanied specialization on different host species.
KW - Afrina wevwlli
KW - Anguina agrostis
KW - Anguina tritici
KW - Diagnostics
KW - Nematode identification
KW - PCR-RFLP
KW - Regulatory nematology
KW - Seed gall nematode
UR - http://www.scopus.com/inward/record.url?scp=0035649582&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035649582&partnerID=8YFLogxK
M3 - Article
C2 - 19265880
AN - SCOPUS:0035649582
VL - 33
SP - 191
EP - 194
JO - Journal of Nematology
JF - Journal of Nematology
SN - 0022-300X
IS - 4
ER -