Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1

T. O. Powers, A. L. Szalanski, P. G. Mullin, T. S. Harris, T. Bertozzi, J. A. Griesbach

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. Each anguinid nematode associated with a unique host produced a unique PCR-RFLP pattern for the ITS1 region. Anguina species that had been synonymized in the past, Anguina agrostis, A. funesta, and A. wevelli (Afrina wevwlli), were readily discriminated. Two undescribed species from northern New South Wales and southeastern South Australia, reported to be vectors of Rathyaibacter toxicus in the disease called "floodplain stagger," were differentiated by a single restriction enzyme, and both could be separated easily from A. funesta, the vector of R. toxicus in annual ryegrass toxicity. Other species differentiated in this study include A. agropyronifloris, A. graminis, A. microlaenae, A. pacificae, and undescrihed species from host species Dactylis glomerata, Agrostis avenacea, Polypogon monospeliensis, Stipa sp., Astrebla pectinata, and Holcus lanatus. Phylogenetic analysis of the ITS1 region suggests that considerable anguinid genetic diversification has accompanied specialization on different host species.

Original languageEnglish (US)
Pages (from-to)191-194
Number of pages4
JournalJournal of Nematology
Volume33
Issue number4
StatePublished - Dec 2001

Keywords

  • Afrina wevwlli
  • Anguina agrostis
  • Anguina tritici
  • Diagnostics
  • Nematode identification
  • PCR-RFLP
  • Regulatory nematology
  • Seed gall nematode

ASJC Scopus subject areas

  • Agronomy and Crop Science

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    Powers, T. O., Szalanski, A. L., Mullin, P. G., Harris, T. S., Bertozzi, T., & Griesbach, J. A. (2001). Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 33(4), 191-194.