Identification of the defects in the hemagglutinin gene of two temperature-sensitive mutants of A/WSN/33 influenza virus

Setsuko Nakajima, Donald J. Brown, Masahiro Ueda, Katsuhisa Nakajima, Akira Sugiura, Asit K. Pattnaik, Debi P. Nayak

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Two temperature-sensitive mutants of WSN influenza virus, ts-61S and ts-134, possess defects in the hemagglutinin (HA) gene. These defects are characterized as a defective intracellular transport of the HA at the nonpermissive temperature and a marked thermolability. The nucleic acid sequences of the HA gene of these two viruses, as well as a series of revertant viruses, were determined. The deduced amino acid sequences demonstrate that the HA of ts-61S varied from the wild type protein by three amino acids while that of ts-134 differed by two residues. For both mutants, analysis of revertant viruses indicated that the phenotype of transport inhibition at the nonpermissive temperature and heat lability were associated with a single amino acid change in the globular portion of the molecule. In the case of ts-61S, the critical change in the HA was the replacement of a serine residue at position 110 with that of a proline. The mutational defect in the HA of ts-134 was due to the substitution of a tyrosine residue at position 159 with that of a histidine residue. Four of five revertants of ts-134 were suppressor revertants, of which some of the compensatory changes did not restore thermostability to the HA.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalVirology
Volume154
Issue number2
DOIs
StatePublished - Oct 30 1986

ASJC Scopus subject areas

  • Virology

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