Identification of the metal associated with the insulin degrading enzyme

Alireza Ebrahim, Frederick G. Hamel, Robert G. Bennett, William C. Duckworth

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Insulin degrading enzyme (IDE) is a thiol-dependent metalloendoprotease that is responsible for initiation of cellular insulin degradation. However, its exact mode of action and the factors controlling it are poorly understood. Since IDE is a metal requiring enzyme, we have examined which metal(s) is(are) endogenously associated with it. Using neutron activation analysis, we studied the metal content of a partially purified enzyme from three different tissues: rat skeletal muscle, rat liver, and human placenta. Our results indicate that zinc and manganese are associated with the enzyme with approximately 10 times more zinc as manganese being present. These results suggest that one or both of these two metals are endogenously associated with this enzyme and are a means of controlling the enzyme's activity.

Original languageEnglish (US)
Pages (from-to)1398-1406
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Dec 31 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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