Identification of two putative novel folate receptor genes in humans and mouse

Ofer Spiegelstein, James D. Eudy, Richard H. Finnell

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Utilizing a 'database mining' strategy to detect novel folate receptors (FR), we identified two potential novel members in the mouse and human. The mouse gene (Folbp3) was sequenced and found to predict a 28.2kDa protein that consists of 244 amino acids that is highly expressed in both the thymus and spleen, suggesting a potential role in the immune system. The human gene (FR-δ) is mapped to chromosome 11q14, and predicts a 27.7kDa protein that is comprised of 241 amino acids. However, expression of the human gene was not detected in 59 samples from both adult and embryonic tissue sources, suggesting a highly restricted spatial/temporal expression pattern, an alternatively spliced variant or an additional FR pseudogene. Using T31 mouse radiation hybrid mapping, Folbp3 was mapped to a region on mouse chromosome 9 that is syntenic to human chromosome 19p13. As the chromosomal locations of Folbp1 murine and Folbp2 genes were previously unknown, we utilized the same approach and mapped both genes to a region of mouse chromosome 7 that is syntenic to the human FR loci on chromosome 11q13. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalGene
Volume258
Issue number1-2
DOIs
StatePublished - Nov 27 2000

Keywords

  • FR-δ
  • Folbp1
  • Folbp2
  • Folbp3
  • Immunity
  • Radiation hybrid mapping

ASJC Scopus subject areas

  • Genetics

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