TY - JOUR
T1 - Ikaros could be a key factor in the maintenance of "B-side" of B-1 cells
AU - Oliveira, Vivian Cristina
AU - Moretti, Nilmar Silvo
AU - Augusto, Leonardo da Silva
AU - Schenkman, Sergio
AU - Mariano, Mario
AU - Popi, Ana Flavia
N1 - Funding Information:
We thank Daniela Teixeira for technical assistance in the cell sorting. This work was supported by FAPESP ( 2008/55526-9 and 2011/50256-6 ).
Publisher Copyright:
© 2015 Elsevier GmbH.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Ikaros, a zinc finger transcription factor, is an important regulator of the hematopoietic system. Several studies have suggested the role of Ikaros in the development, maturation, activation and differentiation of lymphocytes. To elucidate this mechanism, it is important to understand how this transcription factor works in the dichotomy of the hematopoietic system, a topic that remains uncertain. Herein, we investigated the role of Ikaros in the control of the lymphomyeloid phenotype of B-1 lymphocytes. We found that Ikaros, as well as its target genes, are expressed in B-1 cells,. Moreover, Ikaros positively regulates the expression of Flt3, Gfi and Il7r, while it down-regulates PU.1. During the induction of differentiation of B-1 cells toward phagocytes, Ikaros transcription was reduced. Taken together, these data pointed to the relevance of Ikaros in the maintenance of the promiscuous gene profile of B-1 cells. It could be suggested that Ikaros functions as a guardian of B-1 lymphoid pattern, and that its absence directs the differentiation of B-1 cells into phagocytes.
AB - Ikaros, a zinc finger transcription factor, is an important regulator of the hematopoietic system. Several studies have suggested the role of Ikaros in the development, maturation, activation and differentiation of lymphocytes. To elucidate this mechanism, it is important to understand how this transcription factor works in the dichotomy of the hematopoietic system, a topic that remains uncertain. Herein, we investigated the role of Ikaros in the control of the lymphomyeloid phenotype of B-1 lymphocytes. We found that Ikaros, as well as its target genes, are expressed in B-1 cells,. Moreover, Ikaros positively regulates the expression of Flt3, Gfi and Il7r, while it down-regulates PU.1. During the induction of differentiation of B-1 cells toward phagocytes, Ikaros transcription was reduced. Taken together, these data pointed to the relevance of Ikaros in the maintenance of the promiscuous gene profile of B-1 cells. It could be suggested that Ikaros functions as a guardian of B-1 lymphoid pattern, and that its absence directs the differentiation of B-1 cells into phagocytes.
KW - B-1 cells
KW - Ikaros
KW - PU.1
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U2 - 10.1016/j.imbio.2015.06.010
DO - 10.1016/j.imbio.2015.06.010
M3 - Article
C2 - 26141488
AN - SCOPUS:84940450287
SN - 0171-2985
VL - 220
SP - 1232
EP - 1239
JO - Immunobiology
JF - Immunobiology
IS - 11
ER -