IL-1 inhibits β-adrenergic control of cardiac calcium current: Role of L- arginine/nitric oxide pathway

G. J. Rozanski, R. C. Witt

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50 Scopus citations


Modulation of the β-adrenergic control of cardiac L-type Ca2+ current (I(Ca)) by human recombinant interleukin-1β (IL-1) was examined in adult guinea pig ventricular myocytes using the whole cell voltage-clamp technique. I(Ca) was elicited in Cs+-loaded myocytes by depolarizing pulses from a holding potential of -40 mV. Isoproterenol (0.01 and 1 μM) exposed to myocytes pretreated with 1 ng/ml IL-1 evoked a significantly smaller increase in I(Ca) density compared with control cells. This IL-1-mediated decrease in β-responsiveness was usually observed with pretreatment periods of > 1 h and varied as a function of the L-arginine concentration of the pretreatment medium. In addition, it was prevented by 1) IL-1 receptor antagonist, 2) substituting D-arginine for L-arginine, or 3) incubating cells with the nitric oxide synthase inhibitor N(G)-monomethyl-L-arginine. Thus the present data illustrate that IL-1 significantly alters the β-adrenergic control of cardiac Ca2+ channels by cellular mechanisms that involve the activation of nitric oxide synthase. These mechanisms may play a role in altering ventricular function during cytokine-mediated inflammatory processes affecting the heart.

Original languageEnglish (US)
Pages (from-to)H1753-H1758
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number5 36-5
StatePublished - 1994


  • channels
  • cytokines
  • nitric oxide synthase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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