IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

Y. N. Xia, D. E. Burbank, L. Uher, D. Rabussay, J. L. Van Etten

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

Original languageEnglish (US)
Pages (from-to)6075-6090
Number of pages16
JournalNucleic acids research
Volume15
Issue number15
DOIs
StatePublished - Aug 11 1987

ASJC Scopus subject areas

  • Genetics

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