IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

Y. N. Xia; D. E. Burbank; L. Uher; D. Rabussay; J. L. Van Etten

doi:10.1093/nar/15.15.6075

IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

Y. N. Xia, D. E. Burbank, L. Uher, D. Rabussay, J. L. Van Etten

Research output: Contribution to journal › Article › peer-review

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Abstract

A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

Original language	English (US)
Pages (from-to)	6075-6090
Number of pages	16
Journal	Nucleic acids research
Volume	15
Issue number	15
DOIs	https://doi.org/10.1093/nar/15.15.6075
State	Published - Aug 11 1987
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.",

abstract = "A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This {"}star{"} activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor {"}star{"} activity of other restriction endonucleases.",

author = "Xia, {Y. N.} and Burbank, {D. E.} and L. Uher and D. Rabussay and {Van Etten}, {J. L.}",

note = "Funding Information: ACKNOWLEDGMENTS We thank Robert Blakesley, Russel Meints, Les Lane, and Myron Brakke for many helpful discussions. This investigation was supported, in part, by Public Health Service grant GM-32441 from the National Institute of General Medicine and grant DE-ACO2-82ER12086 from the Department of Energy.",

year = "1987",

month = aug,

day = "11",

doi = "10.1093/nar/15.15.6075",

language = "English (US)",

volume = "15",

pages = "6075--6090",

journal = "Nucleic acids research",

issn = "0305-1048",

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TY - JOUR

T1 - IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

AU - Xia, Y. N.

AU - Burbank, D. E.

AU - Uher, L.

AU - Rabussay, D.

AU - Van Etten, J. L.

N1 - Funding Information: ACKNOWLEDGMENTS We thank Robert Blakesley, Russel Meints, Les Lane, and Myron Brakke for many helpful discussions. This investigation was supported, in part, by Public Health Service grant GM-32441 from the National Institute of General Medicine and grant DE-ACO2-82ER12086 from the Department of Energy.

PY - 1987/8/11

Y1 - 1987/8/11

N2 - A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

AB - A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

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JO - Nucleic acids research

JF - Nucleic acids research

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ER -

IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

Abstract

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